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Detection of toxins A/B and isolation of Clostridium difficile and Clostridium perfringens from dogs in Minas Gerais, Brazil BJM
Silva,Rodrigo Otávio Silveira; Santos,Renata Lara Resende; Pires,Prhiscylla Sadanã; Pereira,Luiz Carlos; Pereira,Silvia Trindade; Duarte,Marina Carvalho; Assis,Ronnie Antunes de; Lobato,Francisco Carlos Faria.
The objective of this study was to detect C. difficileA/B toxins and to isolate strains of C. perfringensand C. difficile from diarrheic and non-diarrheic dogs in Brazil. Stool samples were collected from 57 dogs, 35 of which were apparently healthy, and 22 of which were diarrheic. C. difficileA/B toxins were detected by ELISA, and C. perfringensand C. difficilewere identified by multiplex PCR. C. difficileA/B toxins were detected in 21 samples (36.8%). Of these, 16 (76.2%) were from diarrheic dogs, and five (23.8%) were from non-diarrheic dogs. Twelve C. difficile strains (21.1%) were isolated, of which ten were A+B+and two were A-B-. All non-toxigenic strains were isolated from non-diarrheic animals. The binary toxin gene cdtBwas found in one strain,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Nosocomial diarrhea; Small animals; Colitis; Enteritis; Canine.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100019
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