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| Carmona,M.J.C.; Malbouisson,L.M.S.; Pereira,V.A.; Bertoline,M.A.; Omosako,C.E.K.; Le Bihan,K.B.; Auler Jr.,J.O.C.; Santos,S.R.C.J.. |
| The pharmacokinetics of propranolol may be altered by hypothermic cardiopulmonary bypass (CPB), resulting in unpredictable postoperative hemodynamic responses to usual doses. The objective of the present study was to investigate the pharmacokinetics of propranolol in patients undergoing coronary artery bypass grafting (CABG) by CPB under moderate hypothermia. We evaluated 11 patients, 4 women and 7 men (mean age 57 ± 8 years, mean weight 75.4 ± 11.9 kg and mean body surface area 1.83 ± 0.19 m²), receiving propranolol before surgery (80-240 mg a day) and postoperatively (10 mg a day). Plasma propranolol levels were measured before and after CPB by high-performance liquid chromatography. Pharmacokinetic Solutions 2.0 software was used to estimate the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Propranolol; Pharmacokinetics; Cardiopulmonary bypass. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000500008 |
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| Pereira,V.A.; Auler Jr.,J.O.; Carmona,M.J.; Mateus,F.H.; Lanchote,V.L.; Breimer,D.D.; Santos,S.R.C.J.. |
| We describe a new simple, selective and sensitive micromethod based on HPLC and fluorescence detection to measure debrisoquine (D) and 4-hydroxydebrisoquine (4-OHD) in urine for the investigation of xenobiotic metabolism by debrisoquine hydroxylase (CYP2D6). Four hundred µl of urine was required for the analysis of D and 4-OHD. Peaks were eluted at 8.3 min (4-OHD), 14.0 min (D) and 16.6 min for the internal standard, metoprolol (20 µg/ml). The 5-µm CN-reverse-phase column (Shimpack, 250 x 4.6 mm) was eluted with a mobile phase consisting of 0.25 M acetate buffer, pH 5.0, and acetonitrile (9:1, v/v) at 0.7 ml/min with detection at lexcitation = 210 nm and lemission = 290 nm. The method, validated on the basis of measurements of spiked urine, presented 3... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Debrisoquine; 4-hydroxydebrisoquine; HPLC-F; Urinary excretion kinetics; Debrisoquine test validation; CYP2D6. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000500004 |
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| Santos,S.R.; Papini,O.; Omosako,C.E.; Pereira,M.D.; Quintavalle,T.B.G.; Riccio,M.F.; Kurata,A.; Pereira,V.A.; Di-Pietro,G.; Della-Paschoa,O.E.; Danhof,M.; Da-Luz,P.L.. |
| R,S-sotalol, a ß-blocker drug with class III antiarrhythmic properties, is prescribed to patients with ventricular, atrial and supraventricular arrhythmias. A simple and sensitive method based on HPLC-fluorescence is described for the quantification of R,S-sotalol racemate in 500 µl of plasma. R,S-sotalol and its internal standard (atenolol) were eluted after 5.9 and 8.5 min, respectively, from a 4-micron C18 reverse-phase column using a mobile phase consisting of 80 mM KH2PO4, pH 4.6, and acetonitrile (95:5, v/v) at a flow rate of 0.5 ml/min with detection at <FONT FACE="Symbol">l</font>ex = 235 nm and <FONT FACE="Symbol">l</font>em = 310 nm, respectively. This method, validated on the basis of R,S-sotalol measurements in spiked... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: R; S-sotalol racemate HPLC analysis fluorescence detection pharmacokinetics cardiac arrhythmias high-risk patients. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000200006 |
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