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Definition of a diagnostic routine in individuals with inconclusive serology for chagas disease BJID
Picka,Mariele Cristina Modolo; Meira,Domingos Alves; Carvalho,Thaís Batista de; Peresi,Eliana; Marcondes-Machado,Jussara.
Despite the existence of highly sensitive tests, inconclusive serological results are frequent in chronic chagasic infection. This study aimed to define a diagnostic conduct for 30 individuals with inconclusive serology (G3) for chagasic infection assisted at the Outpatient Unit for Infectious and Parasitic Diseases of the Botucatu School of Medicine. Twenty-one individuals with negative serology (G1) and 33 with positive serology (G2) were also studied. Serological methods ELISA, HAI, IFI and immunoblotting TESA-cruzi were used for G1, G2 and G3, and parasitological methods xenodiagnosis, hemoculture and PCR-LIT were used for G2 and G3 individuals. ELISA, HAI and IFI were performed in 5 different blood samples in G2 and G3. TESA-cruzi was carried out only...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chronic chagasic infection; Trypanosoma cruzi; Inconclusive serology; Immunoblotting.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000200012
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Detection of enterotoxins genes in coagulase-negative staphylococci isolated from foods BJM
Cunha,Maria de Lourdes Ribeiro de Souza da; Peresi,Eliana; Calsolari,Regina Adriana Oliveira; Araújo Júnior,João Pessoa.
Staphylococcal food poisoning is caused by ingestion of enterotoxins preformed in the food contaminated essentially through human manipulation or raw material obtained from animals. Although coagulase-positive Staphylococcus aureus is the main agent responsible for food intoxication, some researches emphasise that coagulase-negative staphylococci (CNS) are able to produce staphylococcal enterotoxins and may be a potential cause of food poisoning. In the present study CNS were isolated from foods and the toxigenic capacity of the strains determined. A total of 88 food samples were analysed and 22.7% were positive for CNS strains. Staphylococcal counts ranged from 3.0 x 10² to 1.4 x 10(6) CFU/g or mL of food examined. S. epidermidis predominated among the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Coagulase-negative staphylococci; PCR; Enterotoxins.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000100013
Registros recuperados: 2
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