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| Podio,Maricel; Rodríguez,María P.; Felitti,Silvina; Stein,Juliana; Martínez,Eric J.; Siena,Lorena A.; Quarin,Camilo L.; Pessino,Silvina C.; Ortiz,Juan Pablo A.. |
| In previous studies we reported the identification of several AFLP, RAPD and RFLP molecular markers linked to apospory in Paspalum notatum. The objective of this work was to sequence these markers, obtain their flanking regions by chromosome walking and perform an in silico mapping analysis in rice and maize. The methylation status of two apospory-related sequences was also assessed using methylation-sensitive RFLP experiments. Fourteen molecular markers were analyzed and several protein-coding sequences were identified. Copy number estimates and RFLP linkage analysis showed that the sequence PnMAI3 displayed 2-4 copies per genome and linkage to apospory. Extension of this marker by chromosome walking revealed an additional protein-coding sequence mapping... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Apomixis; Chromosome walking; Gene mapping; Molecular markers. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000500018 |
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