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| Yan,Xu; Souza,Maria Aparecida; Pontes,Marcela Z. R.; Vitolo,Michele; Pessoa Júnior,Adalberto. |
| From analytical to commercial scale, aqueous two-phase systems have their application in the purification, characterization and study of biomaterials. In order to improve the selectivity of the systems, the biospecific affinity ligands were introduced. In the affinity partitioning aqueous two-phase system, have many enzymes been purified. This review discusses the partitioning of some enzymes in the affinity aqueous two-phase systems in regard to the different ligands, including reactive dyes, metal ions and other ligands. Some integration of aqueous two-phase system with other techniques for more effective purification of enzymes are also presented. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Affinity partitioning; Aqueous two-phase systems; Reactive dyes; Ligands; Enzymes. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000400030 |
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| Santos,Carolina Alves dos; Oliveira-Nascimento,Laura; Knirsch,Marcos Camargo; Stephano,Marco Antônio; Pessoa Júnior,Adalberto; Penna,Thereza Christina Vessoni. |
| Ceftazidime is a broad spectrum antibiotic administered mainly by the parenteral route, and it is especially effective against Pseudomonas aeruginosa. The period of time in which serum levels exceed the Minimum Inhibitory Concentration (MIC) is an important pharmacodynamic parameter for its efficacy. One of the forms to extend this period is to administer the antibiotic by continuous infusion, after prior dilution in a Parenteral Solution (PS). The present work assessed the stability of ceftazidime in 5% glucose PS for 24 hours, combined or not with aminophylline, through High Performance Liquid Chromatography (HPLC). The physicochemical evaluation was accompanied by in vitro antimicrobial activity compared MIC test in the 24-hour period. Escherichia coli... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Drug/interactions; Injectables; Parenteral solutions; Ceftazidime/stability; Aminophylline/stability; High Performance Liquid Chromatography/quantitative analysis; Minimum inhibitory concentration (MIC). |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000400013 |
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