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VCP1 PROTEASE DETECTION IN Pochonia chlamydosporia var. CATENULATA STRAIN IMI SD 187 Rev. Protección Veg.
Peteira-Delgado,B; Hidalgo-Díaz,L.
The mode of action of Pochonia as a biological control agent is the penetration by means of the hyphae into the target nematode eggs. The egg shell of nematodes has an outer vitelline membrane composed mainly by proteins. Thus, hyphal penetration is the result of a physic pressure and the specific hydrolytic activity of some enzymes such as proteases and chitinases. The most important protease studied in Pochonia chlamydosporia var. chlamydosporia is VCP1. The aim of this work was to detect and characterize the protease VCP1 in a strain of P. chlamydosporia var. catenulata, a potential biological control agent for root-knot nematodes. The specific activity of this protease was tested on different culture media supplemented with protein inductors using...
Tipo: Journal article Palavras-chave: Nematode parasites; RFLP; Enzymes; Protease.
Ano: 2009 URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1010-27522009000300006
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