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	Provedor de dados Biocell (1) Electron. J. Biotechnol. (1) Autor Jiang,Keji (2) Pi,Yan (2) Gong,Yifu (1) Hou,Rong (1) Lin,Juan (1) Ma,Lingbo (1) Sun,Xiaofen (1) Tang,Kexuan (1) Zhang,Dan (1) Zhang,Fengying (1) Zhu,Houze (1) Mais... Palavra-chave HPLC (1) Optic stalk (1) Potassium acetate (1) RNA isolation (1) Salicylic acid (1) Scylla paramamosain (1) UV-B (1) Mais... Tipo do documento Info:eu-repo/semantics/article (1) Journal article (1) Ano 2012 (1) 2010 (1) País Argentina (1) Chile (1) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Improved isolation of good-quality total RNA from the optic stalk of Mud crab, Scylla paramamosain Electron. J. Biotechnol. Zhu,Houze; Jiang,Keji; Zhang,Fengying; Zhang,Dan; Pi,Yan; Ma,Lingbo. An improved and efficient protocol was developed based on the TaKaRa RNAiso Plus Kit (Code: D9108A) for isolating good-quality total RNA from the optic stalk of mud crab, Scylla paramamosain. The protocol was based on the Trizol method with modifications. The carapace overlapping the optic stalk was retained with RNA in regular protocol. In order to remove the abundant deposition correlative with the carapace which makes the isolation of RNA particularly difficult, 5M potassium acetate solution (pH = 6.0) was added before the precipitation of RNA, and the temperature of RNA deposition was also decreased to -70ºC to ensure the stabilization of RNA. Good-quality total RNA from the optic stalk of S. paramamosain could be easily isolated with this modified... Tipo: Journal article Palavras-chave: Optic stalk; Potassium acetate; RNA isolation; Scylla paramamosain. Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000200005 Examination of camptothecin and 10-hydroxycamptothecin in Camptotheca acuminata plant and cell culture, and the affected yields under several cell culture treatments Biocell Pi,Yan; Jiang,Keji; Hou,Rong; Gong,Yifu; Lin,Juan; Sun,Xiaofen; Tang,Kexuan. Camptothecin and its derivatives are monoterpenoid indole alkaloids exhibiting significant anti-tumor actions. With the aim of improving the production of these pharmaceuticals, the contents of camptothecin and 10-hydroxycamptothecin in different tissues including roots, stems, leaves, young flower buds, opening flowers, fading flowers and seeds from Camptotheca acuminata, were investigated. The young flower buds had the highest alkaloid concentrations (camptothecin, 2.46 mg/g of dry weight; 10-hydroxycamptothecin, 1.41 mg/g of dry weight). Callus showed lower concentrations but it should also be considered as a potential source of these pharmaceuticals. In the present study, the growth rate of Camptotheca acuminata cells in culture did not correlate with... Tipo: Info:eu-repo/semantics/article Palavras-chave: HPLC; Salicylic acid; UV-B. Ano: 2010 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452010000300006 Registros recuperados: 2 Primeira ... 1 ... Última

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