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| Piana,Mariana; Brum,Thiele F. de; Boligon,Aline A.; Alves,Camilla F.S.; Freitas,Robson B. de; Nunes,Letícia T.; Mossmann,Natalia J.; Janovik,Vanessa; Jesus,Roberta S.; Vaucher,Rodrigo A.; Santos,Roberto C.V.; Athayde,Margareth L.. |
| American foulbrood (AFB) is a serious worldwide spreading disease in bees caused by Paenibacillus larvae. Plants extracts are known to decrease or inhibit the growth of these bacteria. The purpose of this study was to evaluate the antimicrobial activity of Calendula. officinalis, Cariniana domestica, and Nasturtium officinale extracts against the P. larvae and to evaluate the toxicity of the extracts in bees. In vitro activity against P. larvae of the extracts was evaluated by micro dilution method and the minimal inhibitory concentrations (MICs) were also determined. The concentrations used in the toxicity test were established based on the MIC values and by the spraying application method. The P. larvae was susceptible to the evaluated crude extract of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antibacterial activity; American foulbrood; Brazilian plants; Paenibacillus larvae. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000201041 |
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| Jesus,Roberta S.; Piana,Mariana; Freitas,Robson B.; Brum,Thiele F.; Alves,Camilla F.S.; Belke,Bianca V.; Mossmann,Natália Jank; Cruz,Ritiel C.; Santos,Roberto C.V.; Dalmolin,Tanise V.; Bianchini,Bianca V.; Campos,Marli M.A.; Bauermann,Liliane de Freitas. |
| Abstract The main objective of this study was to demonstrate the antimicrobial potential of the crude extract and fractions of Chenopodium ambrosioides L., popularly known as Santa-Maria herb, against microorganisms of clinical interest by the microdilution technique, and also to show the chromatographic profile of the phenolic compounds in the species. The Phytochemical screening revealed the presence of cardiotonic, anthraquinone, alkaloids, tannins and flavonoids. The analysis by HPLC-DAD revealed the presence of rutin in the crude extract (12.5 ± 0.20 mg/g), ethyl acetate (16.5 ± 0.37 mg/g) and n-butanol (8.85 ± 0.11 mg/g), whereas quercetin and chrysin were quantified in chloroform fraction (1.95 ± 0.04 and 1.04 ± 0.01 mg/g), respectively. The most... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Amaranthaceae; Antimicrobial potential; Rutin; Quercetin; Chrysin. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000200296 |
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| Lovatto,Naglezi de Menezes; Loureiro,Bruno Bianchi; Bender,Ana Betine Beutinger; Loureiro,Cristiane Bianchi; Goulart,Fernanda Rodrigues; Speroni,Caroline Sefrin; Macagnan,Fernanda Teixeira; Piana,Mariana; Silva,Leila Picolli da. |
| ABSTRACT: Response surface methodology was used to determine optimum conditions for extraction of protein from pumpkin seed meal. A central composite rotational design (CCRD) including independent variables such, pH and sodium trimetaphosphate (STMP) content was used. Maximum yield was obtained at a central point of CCRD, with STMP content and pH of 4% and 4.5, respectively. These conditions resulted in protein yield of 50.04 g of soluble protein from extract/100 g pumpkin seed. The optimization of pH and STMP content allowed obtaining a product with a high protein concentration (62.56 g 100 g-1), digestibility (62.03 g 100 g-1) and concentration of essential amino acids (27.26 g 100 g-1). Regarding the polyphenols concentration, phosphorylated protein... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Amino acids; Central composite rotational design; In vitro digestibility; New ingredient. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020000200751 |
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