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Analytical validation of anti-toxoplasma IgG immunoassays BJID
Souza,Guenael Freire de; Carvalho,Darlene; Pedrosa,William; Franck,Jacqueline; Piarroux,Renaud.
There are often discrepancies when using different methods to measure anti-Toxoplasma gondii IgG levels in patient samples. The diagnostic performance of a chemiluminescent immunoassay (CLIA) and an enzyme-linked fluorescent assay (ELFA) used as confirmatory tests for samples identified as positive or equivocal by an electrochemiluminescent immunoassay (ECLIA) were examined. Cut-off values were those stated by the manufacturer, and Western blot was used to confirm the results of all methods. All samples identified as positive by ECLIA (n = 93) were confirmed as positive by Western blot, as were 14 of the 28 samples identified as equivocal. When these 121 samples were retested, the sensitivities of CLIA and ELFA were 64.4% and 73.8%, respectively. Both...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Toxoplasma; Immunoassay; ECLIA; ELFA.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000600012
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