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| Picco,SJ; Seoane,A; Anchordoquy,JM; Anchordoquy,JP; Rosa,D; Fazzio,LE; Mattioli,GA; Furnus,CC. |
| Zinc is an essential trace element. It has been known that a non adequate level of zinc can alter not only gene expression but also a variety of cellular functions with severe consequences on animal health. Little is known about the role of zinc on the reproductive performance of bovines, especially in oocyte maturation. The objective of the present study was to evaluate the impact of different zinc concentrations added to culture medium, on the DNA integrity of bovine cumulus cells during in vitro maturation of oocytes. Cumulus-oocyte coplexes obtained from an abattoir were cultured in IVM mediums containing 30 (G1), 70 (G2), 110 (G3) and 150 (G4) μg/dl. At the end of IVM, cumulus cells were processed for Comet Assay. Result obtained with the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Zinc; Cumulus cells; Comet assay. |
| Ano: 2008 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1852-62332008000200004 |
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| Boffi,FM; Muriel,MG; López,RA; Ferreira,V; Seoane,A; Picco,SJ. |
| It is well known that two classical consequences of severe exercise are soreness and stiffness. Both consequences develop in the days following severe exercise. The objective of the present work was to establish the association between DNA damage, the energetic metabolism and the effects of the generation of oxygen free radicals in middle gluteal muscle of horses, measured after a severe exercise bout on a treadmill. Four thoroughbreds, ranging in aged from 3 to 4 years old were used for this study. The test consisted in warming-up, severe exercise, and cooling down. Venous blood samples were withdrawn via a catheter from the jugular vein before and at 5 min, 30 min, 45 min and 24 h post-exercise. Muscle biopsy samples were obtained from the middle gluteal... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2007 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1852-62332007000200001 |
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