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| Silva,Adailton Moreira da; Marques-Aguiar,Suely Aparecida; Barros,Regina Maria de Souza; Nagamachi,Cleusa Yoshiko; Pieczarka,Julio Cesar. |
| The genus Uroderma includes two species: U. magnirostrum and U. bilobatum. These species are characterized by their high degree of karyotypic evolution, diverging from most other species of the subfamily Stenodermatinae, which have a lower degree of chromosomic evolution. The present study reports the first banding patterns of U. magnirostrum (G-, C-banding and Ag-NOR) and U. bilobatum (C-banding and Ag-NOR). The chromosomic data in conventional staining of U. magnirostrum (2n = 36, NF = 62) and U. bilobatum (cytotype 2n = 42, NF = 50) are equivalent to that described in the literature. When compared, chromosomal homeologies are found in both karyotypes, as well as differences, confirming that karyotypic evolution in the Uroderma genus is intense. Fission,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cytogenetics; Chromosomal evolution; Phyllostomidae; Uroderma. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000200012 |
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| Silva,Fernando Henrique Ramos; Pieczarka,Julio Cesar; Cardoso,Adauto Lima; Silva,Patrícia Corrêa da; Oliveira,Jonas Alves de; Nagamachi,Cleusa Yoshiko. |
| Cytogenetic studies were carried out on samples of Parapteronotus hasemani, Sternarchogiton preto and Sternarchorhamphus muelleri (Apteronotidae, Gymnotiformes) from the Amazon basin. The first two species exhibited both a 2n = 52 karyotype, but differed in their karyotypic formulae, distribution of constitutive heterochromatin, and chromosomal location of the NOR. The third species, Sternarchorhamphus muelleri, was found to have a 2n = 32 karyotype. In all three species the DAPI and chromomycin A3 staining results were consistent with the C-banding results and nucleolar organizer region (NOR) localization. The 18S rDNA probe confirmed that there was only one pair of ribosomal DNA cistron bearers per species. The telomeric probe did not reveal interstitial... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fluorochromes; FISH; Chromosomal rearrangements; Biodiversity. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000500006 |
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| Milhomem,Susana Suely Rodrigues; Souza,Augusto Cesar Paes de; Nascimento,Aline Lira do; Carvalho Jr.,Jaime Ribeiro; Feldberg,Eliana; Pieczarka,Julio Cesar; Nagamachi,Cleusa Yoshiko. |
| We studied the karyotypes of Hassar cf. orestis and an undescribed Hassar species from the Jarí River and Opsodoras ternetzi, H. orestis and Platydoras cf. costatus from the Xingú River, all with 2n = 58. Constitutive heterochromatin is located in the centromere in most metacentric pairs; in some chromosomes this banding is not present, or it is located on the whole chromosome arm or in the distal regions. The NOR is located on a single biarmed pair at a distal region of the short arm in H. cf. orestis, H. orestis and P. cf. costatus at a distal region of the long arm in O. ternetzi and at a proximal region of the long arm in the Hassar species. In all species (except for Hassar sp.) the CMA3 analysis revealed a rich G-C region coincident with the NOR.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chromosomes; Amazon; Biodiversity; Siluriformes; Banding; Fluorochromes. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200017 |
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| Milhomem-Paixão,Susana Suely Rodrigues; Fascineli,Maria Luiza; Roll,Mariana Matos; Longo,João Paulo Figueiró; Azevedo,Ricardo Bentes; Pieczarka,Julio Cesar; Salgado,Hugo Leonardo Crisóstomo; Santos,Alberdan Silva; Grisolia,Cesar Koppe. |
| Abstract Andirobeira is an Amazonian tree, the seeds of which produce a commercially valuable oil that is used in folk medicine and in the cosmetic industry. Andiroba oil contains components with anti-inflammatory, cicatrizing and insect-repellant actions. However, virtually nothing is known of the safety of this oil for humans. The aim of this work was therefore to investigate the hematotoxicity, genotoxicity and mutagenicity of andiroba oil using the comet and micronucleus assays, and to assess its antioxidant properties and lipidome as a means of addressing safety issues. For the experiments, andiroba oil was administered by gavage for 14 consecutive days in nulliparous female Swiss mice randomly distributed in four groups: negative control and three... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Acute toxicity; Antioxidant activity; Carapa guianensis; Genotoxicity; Lipidomics. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000200248 |
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| Silva,Jéssica Barata da; Suárez,Pablo; Nagamachi,Cleusa Yoshiko; Carter,Timothy Frederick; Pieczarka,Julio Cesar. |
| Plethodontid salamanders of genus Bolitoglossa constitute the largest and most diverse group of salamanders, including around 20% of living caudate species. Recent studies have indicated the occurrence of five recognized species in the Brazilian Amazon Rainforest. We present here the first cytogenetic data of a Brazilian salamander, which may prove to be a useful by contribution to the cytotaxonomy of the genus. Specimens were collected near the "type" locality (Utinga, Belém, PA, Brazil). Chromosomal preparations from duodenal epithelial cells and testes were subjected to Giemsa staining, C-banding and DAPI/CMA3 fluorochrome staining. All specimens showed a karyotype with 13 bi-armed chromosome pairs (2n = 26). Nucleolar Organizer Regions, evidenced by... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Salamanders; Bolitoglossa; Karyotypes; Brazilian Amazon; Cytogenetics. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000400008 |
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| Melo,Karina Motta; Alves,Ingrid Reale; Pieczarka,Julio Cesar; David,José Augusto de Oliveira; Nagamachi,Cleusa Yoshiko; Grisolia,Cesar Koppe. |
| The frequency of spontaneous micronucleus (MN) formation in fish species needs to be determined to evaluate their usefulness for genotoxic biomonitoring. The definition of a good bioindicator takes into account the current knowledge of its metabolic traits as well as other factors including its feeding behavior and relationship to the environment. In this study, we compared the basal frequencies of micronucleated erythrocytes and nuclear abnormalities (NA) among different species of the fish Order Gymnotiformes (Rhamphichthys marmoratus, Steatogenys elegans, Sternopygus macrurus, Parapteronotus hasemani, Gymnotus mamiraua, Gymnotus arapaima, Brachyhypopomus beebei, Brachyhypopomus n. sp. BENN) sampled in several localities of the Eastern Amazon. A baseline... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biomonitoring; Genotoxicity; Gymnotiformes; Amazon region; Fish erythrocytes. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300018 |
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