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Morphometric study of the midgut epithelium in larvae of Diatraea saccharalis Fabricius (Lepidoptera: Pyralidae) Neotropical Entomology
Pinheiro,Daniela O.; Silva,Reinaldo J.; Quagio-Grassiotto,Irani; Gregório,Elisa A..
The sugarcane borer, Diatraea saccharalis Fabricius, has great economical interest as it affects the culture and industrial use of the sugarcane. However, there are few studies concerning the internal morphology of this insect. This work aims to study morphometrically the midgut and the epithelium along their lenght, trying to characterize different regions. Midgut of last instar larvae was divided in three regions: anterior, middle and posterior, and the fragments were processed for light microscopic observation. Histological sections were analyzed in a computerized system concerning the length, width and area of the epithelium, their cells, and the midgut lumen. The obtained data were statistically analyzed by the Kruskal-Wallis test and by multivariate...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Morphometry; Epithelial cell; Insect; Sugarcane borer; Mesenteron.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2003000300012
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Morphological regional differences of epithelial cells along the midgut in Diatraea saccharalis Fabricius (Lepidoptera: Crambidae) larvae Neotropical Entomology
Pinheiro,Daniela O.; Quagio-Grassiotto,Irani; Gregório,Elisa A..
The sugarcane borer, Diatraea saccharalis Fabricius, is a pest to sugarcane and many other crops. This work aims to characterize morphological variability in the epithelial cells (columnar, goblet and regenerative) along the midgut of D. saccharalis larvae. Fragments of the midgut (anterior, middle and posterior regions) were fixed and processed by light and scanning electron microscopy. There are both cytochemical and ultrastructural differences in the morphology of the epithelial cells, depending on their localization along the midgut. The apical surface of columnar cells shows an increase in both number and size of the apical protrusions from the anterior to the posterior midgut regions. There is an increase in the amount of PAS-positive (Periodic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Morphology; Sugarcane borer; Histochemistry; Scanning electron microscopy; Light microscopy.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2008000400009
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Histochemical and ultrastructural evidence of lipid secretion by the silk gland of the sugarcane borer Diatraea saccharalis (Fabricius) (Lepidoptera: Crambidae) Neotropical Entomology
Victoriano,Eliane; Pinheiro,Daniela O.; Gregório,Elisa A..
The silk gland in Lepidoptera larvae is responsible for the silk production used for shelter or cocoon construction. The secretion of fibroin and sericin by the different silk gland regions are well established. There are few attempts to detect lipid components in the insect silk secretion, although the presence of such element may contribute to the resistance of the shelter to wet environment. This study characterizes the glandular region and detects the presence of lipid components in the secretion of the silk gland of Diatraea saccharalis(Fabricius). The silk gland was submitted to histochemical procedure for lipid detection or conventionally prepared for ultrastructural analyses. Lipid droplets were histochemically detected in both the apical cytoplasm...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Secretory cell; Insect; Larva.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2007000500011
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External morphology of Cotesia flavipes (Hymenoptera: Braconidae) during larval development Rev. Bras. Zool.
Pinheiro,Daniela O.; Rossi,Guilherme D.; Cônsoli,Fernando L..
Cotesia flavipes (Cameron, 1891) (Hymenoptera) is a gregarious endoparasitoid used in applied biological control against Diatraea saccharalis (Fabricius, 1794) (Lepidoptera). In this study, we characterize the larval external morphology and the number of instars of C. flavipes. Parasitized larvae of D. saccharalis were sampled from the 1st to the 10th day after parasitism and dissected in an anticoagulant buffer for collection of C. flavipes immatures. Immatures were processed for scanning electron microscopy. Larvae of C. flavipes were prepared in NaOH solution and slide mounted to allow for mandible size measurements. Analysis of measurements of the parasitoid larval mandible size indicated that C. flavipes has three instars. Newly hatched larvae are...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anal vesicle; Biological control; Functional morphology; Ultrastructure.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702010000600022
Registros recuperados: 4
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