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| Ribeiro,Carlos Alexandre Gomes; Pinto,Marcos de Oliveira; Maciel,Talles Eduardo Ferreira; Pastina,Maria Marta; Barros,Everaldo Gonçalves de; Guimarães,Claudia Teixeira. |
| ABSTRACT The development of efficient and low-cost genotyping methods is essential to precise genetic characterization of cultivars. Here, we present a system based on fluorescently labeled universal tail sequence primers (UTSP) to resolve microsatellite (SSR) markers as an alternative for molecular fingerprinting of maize. A set of 20 SSRs using the UTSP presented an average polymorphic information content of 0.84, which provided a probability of random identity ranging from 10−7 to 10−14, and a minimum exclusion power of 99.99998 % in a group of 48 tropical maize single-cross hybrids traded in Brazil. The genetic diversity analysis based on multidimensional scaling explained approximately 28 % of the total variance for the first two coordinates, tending... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Zea mays; Fingerprinting; Genotyping; Cultivar protection. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162017000200163 |
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| Silva,Karla Jorge da; Pastina,Maria Marta; Guimarães,Claudia Teixeira; Magalhães,Jurandir Vieira; Pimentel,Leonardo Duarte; Schaffert,Robert Eugene; Pinto,Marcos de Oliveira; Souza,Vander Fillipe de; Bernardino,Karine da Costa; Silva,Michele Jorge da; Borém,Aluízio; Menezes,Cicero Beserra de. |
| ABSTRACT: Sorghum breeding programs are based predominantly on developing homozygous lines to produce single cross hybrids, frequently with relatively narrow genetic bases. The adoption of complementary strategies, such as genetic diversity study, enables a broader vision of the genetic structure of the breeding germplasm. The purpose of this study was to evaluate the genetic diversity of sorghum breeding lines using structure analysis, principal components (PC) and clustering analyses. A total of 160 sorghum lines were genotyped with 29,649 SNP markers generated by genotyping-by-sequencing (GBS). The PC and clustering analyses consistently divided the R (restorer) and B (maintainer) lines based on their pedigree, generating four groups. Thirty-two B and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Sorghum bicolor; Principal components; Restorer; Maintainer; Heterosis; Hybrid parents. |
| Ano: 2021 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162021000601102 |
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