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| Queiroz,Marisa V. de; Pizzirani-Kleiner,Aline Aparecida; Azevedo,João Lúcio. |
| Pulsed-field gel electrophoresis was used to characterize strains of Aspergillus nidulans with a chromosomal duplication Dp(I-II). Morphologically deteriorated and improved variants of these strains were also analyzed. The electrophoretic karyotype demonstrated that in two duplicated strains (A and B) the 4.2 Mb band, which corresponds to chromosome II, was absent and a new band was observed. Hybridization studies using the uapA (chromosome I) and wA (chromosome II) genes demonstrated that the new band corresponded to chromosome II plus the duplicated segment of chromosome I. The size of the chromosomal duplication was approximately 1.0 Mb. Analysis of the chromosomal bands of a morphologically improved strain showed that the duplicated segment of... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000200009 |
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| Rodrigues,Maria Beatriz Calderan; Andreote,Fernando Dini; Spósito,Marcel Bellato; Aguillar-Vildoso,Carlos Ivan; Araújo,Welington Luiz; Pizzirani-Kleiner,Aline Aparecida. |
| O objetivo deste trabalho foi avaliar a resistência de Guignardia citricarpa aos fungicidas carbendazim e piraclostrobina, por meio de avaliação do crescimento em meio de cultura, decomposição de tecido foliar e produção de corpos de frutificação. Para isso, o fungo G. citricarpa foi isolado de lesões de frutos de laranja (Citrus sinensis), produzidos em área com intensa aplicação de fungicida. Os isolados obtidos foram avaliados quanto à sensibilidade aos fungicidas piraclostrobina e carbendazim, nas dosagens de 0,5, 1 e 2 µg mL-1 de i.a., para se verificar o efeito da pressão de seleção causada pelo uso destes compostos em áreas citrícolas. Embora tenha sido observada redução efetiva no número de estruturas reprodutivas e na decomposição de folhas e... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mancha-preta dos citros; Pinta-preta; Tolerância a fungicidas; Citricultura. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2007000300004 |
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| Giancoli,Ágata Cristiane Huppert; Pizzirani-Kleiner,Aline Aparecida. |
| The Abnc strain of A. nidulans carries the bncA1 gene (binucleated conidia), which induces the formation of binucleate and trinucleate conidia, displaying a chromosome I duplicated area and shifted to the chromosome II (I->II), and bringing forth genetic instability with degenerated sectors. This work has considered in a cytological level the conidiogenesis of the deteriorated variants isolated from the Abnc strain of A. nidulans, observing the event at the level of structural alterations, which composes the conidiophore, and the variations in the number of sterigmata and conidia nuclei. Cytogenetic analyses of conidiogenesis were accomplished in predetermined periods, under Giemsa stain, to observe the nuclei and analysis through the Scanning... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bristle; Conidiophore; Developmental mutants. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162004000400008 |
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| Sebastianes,Fernanda Luiza de Souza; Maki,Cristina Sayuri; Andreote,Fernando Dini; Araújo,Welington Luiz; Pizzirani-Kleiner,Aline Aparecida. |
| The Pink Disease is caused by Erythricium salmonicolor, which attacks broad hosts, such as citrus, coffee, rubber, Eucalyptus spp. and Acacia spp., infecting mainly branches. This disease became a serious problem in Brazil, reducing the citrus production up to 10%. However the genetic diversity and compatibility of the fungus E. salmonicolor from Brazilian citrus plants is not yet evaluated. Therefore, the aims of this study were to evaluate: i) the genetic variability of E. salmonicolor in the São Paulo and Minas Gerais States by the RAPD technique, and ii) the vegetative compatibility between these isolates. After RAPD analysis, six distinct groups were observed without correlation between the isolation site or host species. In the vegetative... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Corticium salmonicolor; RAPD; Pink Disease; Hyphal fusion; Heterokariosis. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162007000200009 |
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| Vicente,Vânia Aparecida; Angelis,Derlene Attili de; Queiróz-Telles Filho,Flávio; Pizzirani-Kleiner,Aline Aparecida. |
| Herpotrichiellaceous fungi, common agents of chromoblastomycosis and phaeohyphomycosis, were searched in samples of rotten wood, leaf littler, bark and soil of the rhizosphere, collected in the Centro Nacional de Pesquisas de Florestas/EMBRAPA, Colombo, PR, Brazil. Morphological analyses of macro, optic and scanning electron microscopy, as well as the determination of the nutritional pattern of the isolated strains were carried out for a taxonomical study. In a total of 17 colonies, 3 isolates (17.6%) were identified as species of medical relevance: Cladophialophora bantiana (Sacc.) de Hoog et al., Fonsecaea pedrosoi (Brumpt) Negroni and Phialophora verrucosa Medlar. The identifications were based on the results of the analyses and on the comparison with... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chromoblastomycosis; Fungal isolation; Herpotrichiellaceae; Phaeohyphomycosis. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000100011 |
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