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Evaluation of phosphorus characterization in broiler ileal digesta, manure, and litter samples: 31P-NMR vs. HPLC NWISRL
Leytem, A.B.; Kwanyuen, P.; Plumstead, P.W.; Maguire, R.O.; Brake, J..
Using 31-phosphorus nuclear magnetic resonance spectroscopy ((31)P-NMR) to characterize phosphorus (P) in animal manures and litter has become a popular technique in the area of nutrient management. To date, there has been no published work evaluating P quantification in manure/litter samples with (31)P-NMR compared to other accepted methods such as high performance liquid chromatography (HPLC). To evaluate the use of (31)P-NMR to quantify myo-inositol hexakisphosphate (phytate) in ileal digesta, manure, and litter from broilers, we compared results obtained from both (31)P-NMR and a more traditional HPLC method. The quantification of phytate in all samples was very consistent between the two methods, with linear regressions having slopes ranging from 0.94...
Tipo: Article Palavras-chave: Chemistry.
Ano: 2008 URL: http://eprints.nwisrl.ars.usda.gov/1268/1/1246.pdf
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Dietary low-phytate mutant-M 955 barley grain alters phytate degradation and mineral digestion in sheep fed high-grain diets NWISRL
Leytem, A.B.; Taylor, J.B.; Raboy, V.; Plumstead, P.W..
Greater production demands for ruminants require increased dietary inclusion of high-energy feeds. Grains and oil seeds are most commonly used to enhance diet energy density. However, use of such feeds proportionally increases the amount of dietary phytate phosphorus (P), which the ruminant may not be able to fully utilise. Our objectives for this study were to determine the extent of phytate degradation and mineral digestion in wethers fed high-grain diets consisting of either a non-mutant or low-phytate mutant barley grain. In two separate experiments, mature Columbia wethers (n = 7) fitted with rumen and duodenal cannulas and Columbia × Polypay wether lambs (n = 8) were individually fed one of two finishing diets formulated with either non-mutant...
Tipo: Article Palavras-chave: Animal health; Animal.
Ano: 2007 URL: http://eprints.nwisrl.ars.usda.gov/1282/1/1259.pdf
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What aspect of dietary modification in broilers controls litter water-soluble phosphorus: Dietary phosphorus, phytase, or calcium? NWISRL
Leytem, A.B.; Plumstead, P.W.; Maguire, R.O.; Kwanyuen, P.; Brake, J..
Tipo: Article Palavras-chave: Chemistry; Mass Import - autoclassified (may be erroneous).
Ano: 2007 URL: http://eprints.nwisrl.ars.usda.gov/45/1/1200.pdf
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Interaction of calcium and phytate in broiler diets: 2. Effects on total and soluble phosphorus excretion NWISRL
Leytem, A.B.; Plumstead, P.W.; Maguire, R.O.; Kwanyuen, P.; Burton, J.W.; Brake, J..
Dietary Ca has been reported to influence the amount of phytate excreted from broilers and affect the solubility of P in excreta. To address the effects of dietary Ca and phytate on P excretion, 12 dietary treatments were fed to broilers from 16 to 21 d of age. Treatments consisted of 3 levels of phytate P (0.10, 0.24, and 0.28%) and 4 levels of Ca (0.47, 0.70, 0.93, and 1.16%) in a randomized complete block design. Feed phytate concentrations were varied by formulating diets with 3 different soybean meals (SBM): a low-phytate SBM, a commercial SBM, and a high phytate Prolina SBM having phytate P concentrations of 0.15 to 0.51%. Fresh excreta was collected from cages during 2 separate 24-h periods; collection I commenced after the start of dietary...
Tipo: Article Palavras-chave: Manure.
Ano: 2008 URL: http://eprints.nwisrl.ars.usda.gov/1280/1/1257.pdf
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Interaction of calcium and phytate in broiler diets: 1. Effects on apparent prececal digestibility and retention of phosphorus NWISRL
Plumstead, P.W.; Leytem, A.B.; Maguire, R.O.; Spears, J.W.; Kwanyuen, P.; Brake, J..
Phytate P utilization from soybean meal (SBM) included in broiler diets has been shown to be poor and highly dependent on dietary Ca intake. However, the effect of Ca on P utilization and on the optimal ratio of Ca to nonphytate P (Ca:NPP) when diets contained varying levels of phytate has not been clearly shown and was the objective of this research. A factorial treatment structure was used with 4 dietary Ca levels from 0.47 to 1.16% and 3 levels of phytate P (0.28, 0.24, and 0.10%). Varying dietary phytate P levels were obtained by utilizing SBM produced from 3 varieties of soybeans with different phytate P concentrations. Ross 508 broiler chicks were fed 1 of 12 diets from 16 to 21 d of age. Excreta were collected from 16 to 17 d and from 19 to 20 d of...
Tipo: Article Palavras-chave: Manure.
Ano: 2008 URL: http://eprints.nwisrl.ars.usda.gov/1279/1/1256.pdf
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