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| Nascimento,Demétrius Fernandes do; Moraes,Manoel Odorico de; Bezerra,Fernando Antônio Frota; Pontes,Andréa Vieira; Uchoa,Célia Regina Amaral; Moraes,Renata Amaral de; Leite,Ismenia Osório; Santana,Gilmara Silva de Melo; Santana,Ana Paula Macedo; Leite,Ana Lourdes Almeida e Silva; Pedrazzoli Júnior,José; Moraes,Maria Elisabete Amaral de. |
| To develop and validate a rapid, specific and highly sensitive method to quantify nimodipine in human plasma using dibucaine as the internal standard (IS). The analyte and IS were extracted from plasma samples by liquid-liquid extraction using hexane-ethyl acetate (1:1 v/v). The chromatographic separation was performed on a Varian® Polaris C18 analytical column (3 μm, 50 x 2.0 mm) and pre-column SecurityguardTM C18 (4.0 x 3.0 mm) with a mobile phase of Acetonitrile-Ammonium acetate 0.02 ml/L (80:20v/v). The method had a chromatographic run time of 4.5 min and linear calibration curve over the range of 0.1- 40 ng/mL (r > 0.9938). The limit of quantification was 100 pg/mL. Acceptable precision and accuracy were obtained for concentrations over the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Nimodipine/determination in plasma; Nimodipine/pharmacokinetic profile; Nimodipine/quantitative analysis; Pharmacokinetics/method validation. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000400008 |
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| Santos,Luciana Kelly Ximenes dos; Cunha,Gilmara Holanda da; Fechine,Francisco Vagnaldo; Pontes,Andréa Vieira; Oliveira,Jonaina Costa de; Bezerra,Fernando Antonio Frota; Moraes,Manoel Odorico de; Moraes,Maria Elisabete Amaral de. |
| The tincture of Operculina alata, popularly known as "tincture of jalapa", is used in Northeast Brazil to treat constipation and encephalic vascular accident, but it has not yet been adequately tested for safety and efficacy. The aim of this study was to evaluate the toxicology and safety of the tincture of O. alata in patients with functional constipation. This was a double-blind, randomized, placebo-controlled clinical trial. The study consisted of three phases: pre-treatment, treatment and post-treatment, each phase with duration of seven days. Arterial pressure, heart rate, body weight, adverse events, hematological, metabolic, liver and kidney functions were monitored. Forty patients were randomized to receive tincture of O. alata and 43 patients to... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Operculina alata/pharmacognosy; Operculina alata/tincture; Operculina alata/toxicological evaluation; Operculina alata/tincture; Operculina alata/safety; Tincture of Jalapa see Operculina alata/tincture. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000300014 |
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