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Validation of in situ hybridisation and histology assays for the detection of the oyster parasite Marteilia refringens ArchiMer
Thebault, Anne; Bergman, S; Pouillot, R; Le Roux, Frédérique; Berthe, Franck.
An in situ hybridisation technique has been developed for the detection of infection in oysters with Marteilia refringens with particular emphasis on light infections or confirmation of suspected cases by means of histology. Although validation of new diagnostic methods is usually achieved by comparison with standard techniques, in our case the sensitivity and specificity of the standard (histology) had not previously been established. Another point to consider is that surveillance and monitoring frequently target populations displaying different levels of prevalence under different field conditions. The objective of our study was to evaluate the sensitivity and specificity values of in situ hybridisation and histology for the detection of M refringens,...
Tipo: Text Palavras-chave: Ostrea edulis; Marteilia refringens; Latent models; Specificity; Sensitivity; Validation; Diagnostic.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-3624.pdf
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