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| Hosseinzadeh,Ali; Feizi,Mohammad Ali Hosseinpour; Kafil,Hossein Samadi; Pouladi,Nasser; Seifi,Morteza; Gavgani,Reyhaneh Ravanbakhsh; Nadergoli,Omid Kheyri; Rostamizadeh,Leila; Montazeri,Vahid; Fakhrjou,Ashraf; Sakhinia,Ebrahim. |
| Many cancer researchers use gene expression analysis for differentiation between tumor and normal cells for diagnostic, prognostic and therapeutic purposes. Most of studies compare either tumor cell lines by normal cell lines or tumor tissue of affected individuals by normal healthy control tissue. But expression of each special gene is unique in different individuals and also in different tissue of same individual. For this reason, here we compare the gene expression levels of SMAD7 and KLF10 in tumor cells and its adjacent normal tissue of breast cancer patients and compared them. For this purpose, a total of 40 tumor and matched tumor-free margin samples were obtained during surgery. The SMAD7 and KLF10 mRNA expression levels in tumor and marginal... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: KLF10; Personalized gene Expression; Real-time PCR; SMAD7; TGFβ. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100317 |
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| Nadergoli,Omid Kheyri; Feizi,Mohammad Ali Hosseinpour; Kafil,Hossein Samadi; Pouladi,Nasser; Hosseinzadeh,Ali; Rostamizadeh,Leila; Montazeri,Vahid; Fakhrjou,Ashraf; Sakhinia,Ebrahim; Seifi,Morteza. |
| ABSTRACT Background: Her-2 and ESR1 genes, that interact in the cell signaling pathway, are the most important molecular markers of breast cancer, which have been amplified or overexpressed in 30% and 70%, respectively. This study was performed to evaluate the gene expression levels of Her-2 and ESR1 genes in tumor cells and its adjacent normal tissue of breast cancer patients and compared them whit clinical-pathological features. Methods: In total, 80 tissue specimens from 40 patients, with an average age of 48.47 years, were examined by Real-time PCR technique, and ultimately evaluated the expression level of Her-2 and ESR1genes. The data were analyzed by REST 2009 V2.0.13 statistical software. Results: HER2 and ESR1 overexpression was identified in 19... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Breast Cancer; Her-2/neu; ESR1; Overexpression; Molecular marker; Real-time PCR. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100317 |
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| Nadergoli,Omid Kheyri; Feizi,Mohammad Ali Hosseinpour; Kafil,Hossein Samadi; Pouladi,Nasser; Hosseinzadeh,Ali; Rostamizadeh,Leila; Montazeri,Vahid; Fakhrjou,Ashraf; Sakhinia,Ebrahim; Seifi,Morteza. |
| ABSTRACT Background: Her-2 and ESR1 genes, that interact in the cell signaling pathway, are the most important molecular markers of breast cancer, which have been amplified or overexpressed in 30% and 70%, respectively. This study was performed to evaluate the gene expression levels of Her-2 and ESR1 genes in tumor cells and its adjacent normal tissue of breast cancer patients and compared them whit clinical-pathological features. Methods: In total, 80 tissue specimens from 40 patients, with an average age of 48.47 years, were examined by Real-time PCR technique, and ultimately evaluated the expression level of Her-2 and ESR1genes. The data were analyzed by REST 2009 V2.0.13 statistical software. Results: HER2 and ESR1 overexpression was identified in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Breast Cancer; Her-2/neu; ESR1; Overexpression; Molecular marker; Real-time PCR. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100304 |
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