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| Mari, Jocelyne; Poulos, Bonnie; Lightner, Donald; Bonami, Jean-robert. |
| The single-stranded genomic RNA of Taura syndrome virus (TSV) is 10205 nucleotides in length, excluding the 3' poly(A) tail, and contains two large open reading frames (ORFs) that are separated by an intergenic region of 207 nucleotides. The ORFs are flanked by a 377 nucleotide 5' untranslated region (UTR) and a 226 nucleotide 3' UTR followed by a poly(A) tail. The predicted amino acid sequence of ORF1 revealed sequence motifs characteristic of a helicase, a protease and an RNA-dependent RNA polymerase, similar to the non-structural proteins of several plant and animal RNA viruses. In addition, a short amino acid sequence located in the N-terminal region of ORF1 presented a significant similarity with a baculovirus IAP repeat (BIR) domain of inhibitor of... |
| Tipo: Text |
Palavras-chave: Genomic characterization; Taura syndrome; Virus; Shrimp disease; Penaeidae. |
| Ano: 2002 |
URL: https://archimer.ifremer.fr/doc/2002/publication-715.pdf |
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| Poulos, Bonnie; Tang, K; Pantoja, C; Bonami, Jean-robert; Lightner, D. |
| The causative agent of myonecrosis affecting cultured Penaeus vannamei in Brazil was demonstrated to be a virus after purification of the agent from infected shrimp tissues. Purified viral particles were injected into specific pathogen-free P. vannamei, resulting in a disease that displayed the same characteristics as those found in the original shrimp used for purification. The virus was named infectious myonecrosis virus (IMNV). The viral particles were icosahedral in shape and 40 nm in diameter, with a buoyant density of 1(.)366 g ml(-1) in caesium chloride. The genome consisted of a single, double-stranded (dsRNA) molecule of 7560 bp. Sequencing of the viral genome revealed two non-overlapping open reading frames (ORFs). The 5' ORF (ORF 1, nt 136-4953)... |
| Tipo: Text |
Palavras-chave: Purification; Myonecrosis; Virus; Shrimp; Penaeid. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1504.pdf |
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