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Modélisation basée sur la théorie des budgets d’énergie dynamiques (DEB) de la cinétique de bioaccumulation des toxines paralysantes (PST) par l’huître creuse Crassostrea gigas ArchiMer
Pousse, Emilien.
The oyster Crassostrea gigas is one of the most commercialized molluscs in the world. It is a very cosmopolitan species that has been disseminated by humans and is susceptible to various agents such as pathogens (bacteria, viruses), parasites, xenobiotics and/or toxic micro-algae. As a filter-feeder, it may accumulate phyco- toxins while feeding on harmful algal blooms (HAB), including paralytic shellfish toxins (PST). To protect human health shellfisheries harvesting are closed dur- ing HAB, impacting the aquaculture industry. This PhD work aims to develop a Dynamic Energy Budget (DEB) based model to describe kinetics of PST accumulation and detoxification in C. gigas. Interest of coupling bio-accumulation to DEB model lies in the inclusion of...
Tipo: Text Palavras-chave: Budgets d’Énergie Dynamiques; Toxines paralysantes; Pacific oyster; Cinétiques d’accumulation; Écotoxicologie; Dynamic Energy Budget; Paralytic Shellfish Toxins; Pacific oyster; Accumulation kinetics; Ecotoxicology.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00456/56792/58631.pdf
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Sources of paralytic shellfish toxin accumulation variability in the Pacific oyster Crassostrea gigas ArchiMer
Pousse, Emilien; Flye-sainte-marie, Jonathan; Alunno-bruscia, Marianne; Hegaret, Helene; Jean, Fred.
This study was designed to assess the contribution of feeding behavior to inter-individual variability of paralytic shellfish toxin (PST) accumulation in the Pacific oyster Crassostrea gigas. For this purpose 42 oysters were exposed for 2 days to non-toxic algae and then for 2 other days to the PST producer Alexandrium minutum. Individual clearance rate (CR) of oysters was continuously monitored over the 4 days using an ecophysiological measurement system. Comparison of CR values when exposed to toxic and non toxic algae allowed to estimate a clearance rate inhibition index (CRII). Toxin concentration of oysters was quantified at the end of the experiment. These data allowed to estimate the toxin accumulation efficiency (TAE) as the ratio of toxin...
Tipo: Text Palavras-chave: Alexandrium minutum; Paralytic shellfish poisoning; Accumulation; Clearance rate; Feeding behavior; Pacific oyster.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00416/52789/53688.pdf
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Modelling paralytic shellfish toxins (PST) accumulation in Crassostrea gigas by using Dynamic Energy Budgets (DEB) ArchiMer
Pousse, Emilien; Flye-sainte-marie, Jonathan; Alunno-bruscia, Marianne; Hegaret, Helene; Rannou, Eric; Pecquerie, Laure; Marques, Goncalo M.; Thomas, Yoann; Castrec, Justine; Fabioux, Caroline; Long, Marc; Lassudrie, Malwenn; Hermabessiere, Ludovic; Amzil, Zouher; Soudant, Philippe; Jean, Fred.
As other filter-feeders, Crassostrea gigas can concentrate paralytic shellfish toxins (PST) by consuming dinoflagellate phytoplankton species like Alexandrium minutum. Intake of PST in oyster tissues mainly results from feeding processes, i.e. clearance rate, pre-ingestive sorting and ingestion that are directly influenced by environmental conditions (trophic sources, temperature). This study aimed to develop a mechanistic model coupling the kinetics of PST accumulation and bioenergetics in C. gigas based on Dynamic Energy Budget (DEB) theory. For the first time, the Synthesizing Units (SU) concept was applied to formalize the feeding preference of oysters between non-toxic and toxic microalgae. Toxin intake and accumulation were both dependent on the...
Tipo: Text Palavras-chave: Alexandrium minutum; Paralytic shellfish toxins (PST); Dynamic Energy Budget (DEB); Modelling; Pacific oyster.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00455/56662/58407.pdf
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A DEB model to predict accumulation and detoxification of paralytic shellfish toxins by the Japanese Oyster (Crassostrea gigas) ArchiMer
Pousse, Emilien; Jean, Frédéric; Alunno-bruscia, Marianne; Flye Sainte Marie, Jonathan.
France being the largest consumer of oysters in Europe, oyster farming is deeply rooted in French heritage. The Japanese oyster (Crassostrea gigas) is the oyster species the most exploited in France, and in the world. Due to filter-feeding, these bivalves are sensitive to toxic algal blooms. Although not always lethal, toxic algae can affect oyster physiology and make it unfit for human consumption. Phytoplankton toxins can be classified in several groups: amnesic, neurotoxic, diarrhetic and paralytic. For the latter group, saxitoxins are synthesized by the microalgae of genus Alexandrium that can accumulate bivalve tissues. In recent years, number of studies have been performed on the interactions between C. gigas and saxitoxins. In order to better...
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Ano: 2015 URL: http://archimer.ifremer.fr/doc/00307/41859/41100.pdf
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