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| Pacheco,W. A.; Genovez,M. E.; Pozzi,C. R.; Silva,L. M. P.; Azevedo,S. S.; Did,C. C.; Piatti,R. M.; Pinheiro,E. S.; Castro,V.; Miyashiro,S.; Gambarini,M. L.. |
| This paper aimed to determine the excretion period of B19 vaccine strain during a complete reproductive cycle (from estrus synchronization, artificial insemination, pregnancy and until 30 days after parturition) of dairy cows from 3 to 9 years old that were previously vaccinated from 3 to 8 months. Three groups were monitored with monthly milk and urine collection during 12 months: G1 with seven cows from 3 to 4 years old; G2 with three cows from 5 to 6 years old; and G3 with four cows from 7 to 9 years old. Urine and milk samples were submitted to bacteriological culture and urine and PCR reactions for detection of Brucella spp. and PCR-multiplex for B19 strain identification. Ring test (RT) was also performed in the milk samples, and serum samples were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovine brucellosis; Vaccination; B19 vaccine; Excretion. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200022 |
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