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Otolith growth in trout Oncorhynchus mykiss: supply of Ca2+ and Sr2+ to the saccular endolymph ArchiMer
Payan, P; Borelli, G; Priouzeau, F; De Pontual, Helene; Boeuf, G; Mayer Gostan, N.
Kinetic and pharmacological characteristics of Ca2+ fluxes across the saccular epithelium of trout were studied using a perfused isolated inner ear. Ca-45(2+) influx from the Ringer solution to the endolymph was 3-4 nmoles h(-1) mu(-1) endolymph, which corresponds to a global turnover rate of the endolymph calcium of 200% h(-1). Ca2+ entry into the proximal endolymph was faster than into the distal fluid. Net Ca2+ movement across the saccular epithelium depended on the direction and intensity of the chemical gradient of calcium between the Ringer solution and the endolymph. Increasing the calcium concentration in the Ringer solution up to 4.4 mmol l(-1) provoked an accumulation of Ca2+ in both proximal and distal endolymphs, and equilibrium was reached...
Tipo: Text Palavras-chave: Inner ear; Perfusion; Otolith; Endolymph; Flux; Calcium; Oncorhynchus mykiss; Trout.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/2002/publication-416.pdf
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Characterization and variations of organic parameters in teleost fish endolymph during day-night cycle, starvation and stress conditions ArchiMer
Guibbolini, M; Borelli, G; Mayer Gostan, N; Priouzeau, F; De Pontual, Helene; Allemand, D; Payan, P.
The aim of the present work was to examine the modifications of the organic composition of fish endolymph under environmental conditions (day-night cycle, starvation and Cl-2-Stress) known to modify otolith growth. Endolymph electrophoretic patterns were compared. An antibody raised against the trout otolith organic matrix allowed examining the variations of organic matrix precursors in the endolymph under the above conditions. Western blot analysis showed bands around 60-80 kDa. A 50% decrease of immunolabelling was observed during the night whereas increases were seen after starvation (factor 3) or stress (factor 2) suggesting that these variations could be related to the organic matrix deposit. A factor retarding in vitro CaCO3 crystallization (FRC) was...
Tipo: Text Palavras-chave: Turbot; Trout; Stress; Starvation; Day night cycle; Organic matrix; Otolith calcification; In vitro crystallization; Endolymph.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1875.pdf
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