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| Petrillo,Caroline Pereira; Carneiro,Newton Portilho; Purcino,Antônio Álvaro Corsetti; Carvalho,Carlos Henrique Siqueira; Alves,José Donizeti; Carneiro,Andréa Almeida. |
| The objective of this work was to develop a genetic transformation system for tropical maize genotypes via particle bombardment of immature zygotic embryos. Particle bombardment was carried out using a genetic construct with bar and uidA genes under control of CaMV35S promoter. The best conditions to transform maize tropical inbred lines L3 and L1345 were obtained when immature embryos were cultivated, prior to the bombardment, in higher osmolarity during 4 hours and bombarded at an acceleration helium gas pressure of 1,100 psi, two shots per plate, and a microcarrier flying distance of 6.6 cm. Transformation frequencies obtained using these conditions ranged from 0.9 to 2.31%. Integration of foreign genes into the genome of maize plants was confirmed by... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Zea mays; Ammonium glufosinate; Beta-glucuronidase; Immature embryos; Phosphinotricin acetyl transferase; Plant transformation. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008000300012 |
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