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| Berenschot,Amanda S.; Zucchi,Maria I.; Tulmann-Neto,Augusto; Quecini,Vera. |
| Traditionally, mutagenesis has been used to introduce novel genetic variability in ornamental crops. More recently, it has become a powerful tool in gene discovery and functional analyses in reverse genetics approaches. The present work aimed to compare the efficiency of physical and chemical agents in generating mutant populations of petunia. We have indirectly evaluated the genomic damage by analyzing developmental characteristics of the plantlets derived from treated seeds employing gamma radiation at 0, 20, 40, 60, 80 and 100 Gy and the alkylating agent ethyl-methanesulfonate (EMS) at 0, 0.05, 0.1, 0.15, 0.2 and 0.25% (v/v). Gamma rays and EMS caused developmental defects and decreased seedling viability in plants obtained from the mutagenized seeds.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: EMS; Gamma irradiation; Genomic damage; Mutagenesis; Reverse genetics. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202008000200002 |
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| Quecini,Vera; Torres,Gisele A.M.; Rosa Jr,Vicente E. de; Gimenes,Marcos A.; Machado,Jeanne B. de M.; Figueira,Antonio V. de O.; Benedito,Vagner; Targon,Maria Luisa P.N.; Cristofani-Yaly,Mariângela. |
| Plant hormones play a crucial role in integrating endogenous and exogenous signals and in determining developmental responses to form the plant body throughout its life cycle. In citrus species, several economically important processes are controlled by phytohormones, including seed germination, secondary growth, fruit abscission and ripening. Integrative genomics is a powerful tool for linking newly researched organisms, such as tropical woody species, to functional studies already carried out on established model organisms. Based on gene orthology analyses and expression patterns, we searched the Citrus Genome Sequencing Consortium (CitEST) database for Expressed Sequence Tags (EST) consensus sequences sharing similarity to known components of hormone... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Defense responses; Development; Plant growth regulators. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500002 |
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| Quecini,Vera. |
| Studies employing model species have elucidated several aspects of photoperception and light signal transduction that control plant development. However, the information available for economically important crops is scarce. Citrus genome databases of expressed sequence tags (EST) were investigated in order to identify genes coding for functionally characterized proteins responsible for light-regulated developmental control in model plants. Approximately 176,200 EST sequences from 53 libraries were queried and all bona fide and putative photoreceptor gene families were found in citrus species. We have identified 53 orthologs for several families of transcriptional regulators and cytoplasmic proteins mediating photoreceptor-induced responses although some... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cryptochrome; Data mining; Light signaling; Phototropin; Phytochrome. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500007 |
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| Mulato,Bruno Mello; Möller,Milene; Zucchi,Maria Imaculada; Quecini,Vera; Pinheiro,José Baldin. |
| The objectives of this work were to investigate the genetic variation in 79 soybean (Glycine max) accessions from different regions of the world, to cluster the accessions based on their similarity, and to test the correlation between the two types of markers used. Simple sequence repeat markers present in genomic (SSR) and in expressed regions (EST-SSR) were used. Thirty SSR primer-pairs were selected (20 genomic and 10 EST-SSR) based on their distribution on the 20 genetic linkage groups of soybean, on their trinucleotide repetition unit and on their polymorphism information content. All analyzed loci were polymorphic, and 259 alleles were found. The number of alleles per locus varied from 2-21, with an average of 8.63. The accessions exhibit a... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Glycine max; Molecular marker; Plant breeding; Plant germplasm; Polymorphism information content. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2010000300007 |
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