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Genetic diversity in Brazilian sweet potato (Ipomoea batatas (L.) Lam., Solanales, Convolvulaceae) landraces assessed with microsatellite markers Genet. Mol. Biol.
Veasey,Elizabeth Ann; Borges,Aline; Rosa,Mariana Silva; Queiroz-Silva,Jurema R.; Bressan,Eduardo de Andrade; Peroni,Nivaldo.
We used simple sequence repeat (SSR) markers to investigate the genetic diversity of 78 sweet potato (Ipomoea batatas) accessions (58 landraces and 20 putative clones) from traditional agricultural households from 19 local communities in the Vale do Ribeira, São Paulo, Brazil. Eight SSR loci were assessed using 6% (w/v) polyacrylamide gels stained with silver nitrate and the accessions genotyped considering the presence or absence of bands. The results were subjected to analysis of molecular variance (AMOVA), and cluster and principal coordinate analyses. Spatial structure was assessed using Mantel's test to compare genetic and geographic distances. Each primer pair generated between three and ten clearly scorable polymorphic fragments. Cluster analyses...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genetic variability; Landraces; SSR; Traditional agriculture; Vale do Ribeira.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400020
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Genetic characterization of cassava ( Manihot esculenta ) landraces in Brazil assessed with simple sequence repeats Genet. Mol. Biol.
Siqueira,Marcos V. B. M.; Queiroz-Silva,Jurema R.; Bressan,Eduardo A.; Borges,Aline; Pereira,Kayo J. C.; Pinto,José G.; Veasey,Elizabeth Ann.
Based on nine microsatellite loci, the aim of this study was to appraise the genetic diversity of 42 cassava ( Manihot esculenta ) landraces from selected regions in Brazil, and examine how this variety is distributed according to origin in several municipalities in the states of Minas Gerais, São Paulo, Mato Grosso do Sul, Amazonas and Mato Grosso. High diversity values were found among the five above-mentioned regions, with 3.3 alleles per locus on an average, a high percentage of polymorphic loci varying from 88.8% to 100%, an average of 0.265 for observed heterozygosity and 0.570 for gene diversity. Most genetic diversity was concentrated within the regions themselves ( H S = 0.52). Cluster analysis and principal component based scatter plotting...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genetic diversity; Microsatellites; SSR markers; Traditional farming.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100015
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