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| Sánchez-Olate,Manuel; Ríos,Darcy; Rodríguez,Roberto; Materán,María Elena; Pereira,Guillermo. |
| Se analizó la duración del efecto revigorizante de podas severas de avellano europeo (Corylus avellana L.) cv. Negretta adulto. Se evaluó la estimulación y la tasa de proliferación in vitro, sobre explantos resultantes de la estimulación de varetas obtenidas desde plantas adultas sin poda (T1), brotes epicórmicos de plantas sin poda (T2), plantas podadas una sola vez (T3) y plantas podadas dos veces (T4). En una primera etapa, se evaluó la respuesta a la estimulación climática utilizando varetas de entre 25 y 35 cm de largo y diámetros basales de entre 0,5 y 1,5 cm, obtenidas de plantas T1, T2, T3 y T4. En una segunda etapa, se evaluó la tasa de proliferación in vitro de explantos originados por la estimulación climática. Los resultados se analizaron... |
| Tipo: Journal article |
Palavras-chave: Avellano; Cultivo in vitro; Capacidad morfogénica; Tejido adulto. |
| Ano: 2004 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0365-28072004000400002 |
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| Sánchez-Olate,Manuel; Sáez,Patricia; Cartes,Priscila; Alvarez,Carolina; Ríos,Darcy. |
| From in vitro cultured adult material of Corylus avellana L. cv. Negretta, adventitious rooting of microshoots was evaluated. Rhizogenic induction mediated by two strains of wild-type Agrobacterium rhizogenes (A477 and A478) and indolbutyric acid (IBA) were compared under two light conditions (16:8 h photoperiod and complete darkness). The results indicate that in the 16:8 h photoperiod induction, the rooting rate with IBA (90%) was significantly higher than that obtained with the strain A477 of A. rhizogenes (67.7%), while with the strain A478 no statistically significant difference was obtained for the same variable (75%). On the other hand, under complete darkness, rooting mediated by IBA (90%) significantly surpassed the results obtained with both... |
| Tipo: Journal article |
Palavras-chave: Rooting; European hazelnut. |
| Ano: 2009 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392009000100013 |
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| Avilés,Fabiola; Ríos,Darcy; González,Ricardo; Sánchez-Olate,Manuel. |
| To define preliminary aspects of walnut (Juglans regia L.) callogenesis in indirect propagation systems, this work analyzed callogenic induction in adult leaves of walnut in broadleaf trees (BTM), Murashige and Skoog (MS) Driver Kuniyuki Walnut (DKW) and Woody Plant (WPM) media. Leaf segments of 1 cm², previously sterilized, were placed in each culture medium, supplemented with 21.4 µM naphtalene acetic acid (NAA) and 8.8 µM 6-benzylaminopurine (BAP). A completely random design was used; the experimental unit was a Petri dish with six callus explants. Each treatment was composed of seven Petri dishes. Callogenesis (%), nodular calli (%) and histology of calli explants were evaluated at 4 weeks by staining 10 µm slices with safranine-fastgreen. There were... |
| Tipo: Journal article |
Palavras-chave: Auxins; Citoquinines; Walnut; Callus; Leaves. |
| Ano: 2009 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392009000300020 |
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| Sánchez-Olate,Manuel; Sáez,Patricia; Ríos,Darcy. |
| The in vitro introduction of adult walnut (Juglans regia L.) tissue represents an opportunity to clone elite genotypes whose selection occurs in advanced ontogenic states. With the purpose of developing a protocol to allow mass propagation of valuable genotypes from adult material, a comparison was made between two root induction systems of walnut microshoots of the fourth subculture of adult walnut tissue of an in vitro introduction program previously reinvigorated through traditional grafting. Rhizogenic induction by indole-3-butyric acid (IBA) and Agrobacterium rhizogenes was used. The rhizogenic process was analyzed in two phases for both auxinic (T1: 3 mg L-1 IBA; T2: 5 mg L-1 IBA) and A. rhizogenes inductions (T3: A-477; T4: A-478). The first phase... |
| Tipo: Journal article |
Palavras-chave: Rooting; Microshoots; Adult material. |
| Ano: 2009 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392009000200019 |
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