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REYES-ZAMBRANO,SHEILA JAZMÍN; LECONA-GUZMÁN,CARLOS ALBERTO; BARREDO-POOL,FELIPE ALONSO; AMBROSIO CALDERÓN,JOSÉ DOLORES; ABUD-ARCHILA,MIGUEL; RINCÓN-ROSALES,REINER; RUIZ-VALDIVIEZO,VÍCTOR MANUEL; GUTIÉRREZ-MICELI,FEDERICO ANTONIO. |
Current protocols for Agave americana L. micropropagation have limited commercial application due to the low number of plants produced by explant. Indirect organogenesis could be an alternative, however is necessary to optimize plant growth regulators for plantlet number maximization. The objective of this work was to optimize 2,4-dicholorophenoxyacetic acid (2,4-D) and 6-benzyl adenine (BA) concentrations on the induction of A. americana embryogenic callus from apical meristem as explant for maximized the number of shoots per callus using a response surface experimental design. MS medium containing 30 g l-1 sucrose amended with 0.11,0.18, 0.45 or 2.26 μM 2,4-D, and 11.0, 22.0, 38.2 or 44.0 μM BA was used. Nine treatments with three... |
Tipo: Journal article |
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Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-66432016000100014 |
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RINCÓN-ROSALES,REINER; RUÍZ-VALDIVIEZO,VÍCTOR M; MONTES-MOLINA,JOAQUÍN A; GUTIÉRREZ-MICELI,FEDERICO A; DENDOOVEN,LUC. |
The tropical legume Acaciella angustissima inoculated with or without Sinorhizobium mexicanum ITTG R7T strain was cultivated in an aluminium (Al) spiked soil to study its tolerance towards this metal. Seedlings of A. angustissima were grown in soil with 0, 2, 4 or 6 mg Al kg-1. The effect of Al and inoculation on growth, nodulation, and nitrogen and tannins content in the plants was monitored. Height and total and dry root weight of plantlets decreased significantly when soil was spiked with Al, but increased when inoculated. Al decreased the number of nodules. Inoculum increased total N and tannin content of the plantlets. It was found that Al reduced the growth of the tropical leguminous N2-fixing shrub A. angustissima, while inoculation with S.... |
Tipo: Journal article |
Palavras-chave: Aluminium tolerance; Plant growth; Nodulation; Nitrogen and tannins content; Sinorhizobium mexicanum ITTG R7T; Soil. |
Ano: 2011 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-66432011000200009 |
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