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| Rabbani,Mohammed; Bagherinejad,Mohammad Reza; Sadeghi,Hamid MirMohammad; Shariat,Ziaedin Samsam; Etemadifar,Zahra; Moazen,Fatemeh; Rahbari,Manizheh; Mafakher,Ladan; Zaghian,Saeideh. |
| The purpose of the present study was to screen and identify the lipase-producing microorganisms from various regions of Iran. Samples collected from hot spring, Persian Gulf, desert area and oil-contaminated soil, were analyzed for thermophilic extracellular-lipase producing organisms. Six strains with high activity on rhodamine B plates were selected for chemical identification and further study. Among these isolated bacteria, four strains show higher activity in pH-Stat method at 55 °C. These strains were identified by PCR amplification of 16s rRNA genes using universal primers. Fermentation increased the activity up to 50%. The growth medium, designed for lipase production, increased the activity up to 4.55 folds. The crude supernatant of ZR-5 after... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Extracellular lipase; PH-stat method; Thermophilic lipase; Fermentation. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400013 |
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