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Rajoka,Muhammad Ibrahim. |
The influence of carbon and nitrogen sources on the production of exo-glucanase was investigated. The enzyme production was variable according to the carbon or nitrogen source used. Levels of beta-cellobiohydrolase (CBH) were minimal in the presence of even low concentrations of glucose. Enzyme production was stimulated by other carbohydrates and thus is subject to carbon source control by easily metabolizable sugars. In Dubos medium, on cellobiose, the cellobiohydrolase titres were 2-to 110-fold higher with cells growing on monomeric sugars and 2.7 times higher than cells growing on other disaccharides. alpha-Cellulose was the most effective inducer of beta-cellobiohydrlase and filter paperase (FPase) activities, followed by kallar grass straw.... |
Tipo: Journal article |
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Ano: 2004 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582004000300007 |
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Rajoka,Muhammad Ibrahim; Khan,Sana. |
Production of β-xylosidaseby a cycloheximide and 2-deoxy-D-glucose-resistant mutant of Kluyveromyces marxianus PPY125 was studied when cultured on growth media containing galactose, glucose, xylose, cellobiose, sucrose and lactose as carbon sources. Xylose, cellobiose, lactose and sucrose were the key substrates. Both K. marxianus PPY125 and its mutant (M 125) supported maximum β-xylosidase specific product yield (Y P/X) following growth on xylose. Basal level of activity was observed in non-induced cultures grown on glucose. The mutant produced 1.5 to 2-fold more β-xylosidase than that produced by the wild cells. Synthesis of β-xylosidase was regulated by an induction mechanism in both wild and mutant cells. Addition of... |
Tipo: Journal article |
Palavras-chave: Enthalpy; Entropy; Enzyme kinetics; Fermentation; Induction; Thermodynamics. |
Ano: 2005 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000200007 |
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