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Aptamers as a promising approach for the control of parasitic diseases BJID
Ospina-Villa,Juan David; Zamorano-Carrillo,Absalom; Castañón-Sánchez,Carlos A.; Ramírez-Moreno,Esther; Marchat,Laurence A..
ABSTRACT Aptamers are short single-stranded RNA or DNA oligonucleotides that are capable of binding various biological targets with high affinity and specificity. Their identification initially relies on a molecular process named SELEX (Systematic Evolution of Ligands by EXponential enrichment) that has been later modified in order to improve aptamer sensitivity, minimize duration and cost of the assay, as well as increase target types. Several biochemical modifications can help to enhance aptamer stability without affecting significantly target interaction. As a result, aptamers have generated a large interest as promising tools to compete with monoclonal antibodies for detection and inhibition of specific markers of human diseases. One aptamer-based drug...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aptamer; Parasite control; Protozoan parasite; SELEX strategy.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000600610
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Effect of ultrasound on survival and growth of Escherichia coli in cactus pear juice during storage BJM
Cruz-Cansino,Nelly del Socorro; Reyes-Hernández,Isidro; Delgado-Olivares,Luis; Jaramillo-Bustos,Diana Pamela; Ariza-Ortega,José Alberto; Ramírez-Moreno,Esther.
Abstract The aim of this study was to investigate the effectiveness of ultrasound as a conservation method for the inactivation of Escherichia coli inoculated into cactus pear juices (green and purple). Total soluble solids, pH, titratable acidity, and the kinetics of E. coli in cactus pear juices treated by ultrasound (60%, 70%, 80% and 90% amplitude levels for 1, 3 and 5 min) were evaluated over 5 days. Total inactivation was observed in both fruit juices after 5 min of ultrasound treatment at most amplitude levels (with the exception of 60% and 80%). After one and two days of storage, the recovery of bacteria counts was observed in all cactus pear juices. Ultrasound treatment at 90% amplitude for 5 min resulted in non-detectable levels of E. coli in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ultrasound; Growth; Escherichia coli; Cactus pear juice; Storage.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200431
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