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| Brum,Francis Maria Rocho de; Randi,Aurea Maria. |
| Rumohra adiantiformis (Forst.) Ching is a fern (Dryopteridaceae) used in floral arrangements. Spores sterilized in 15% (v/v) solution of commercial sodium hypochlorite for 10 minutes and unsterilized spores were plunged in liquid nitrogen and held for 15 minutes and for 90 days. After the cryogenic treatments, spores were taken out of liquid nitrogen and rapidly thawed out in a water bath or slowly at room temperature and were cultured in Mohr's mineral solution as modified by Dyer, kept at 25 ± 2 ºC and a 16-hours photoperiod. Statistical differences were not observed in the germination of unsterilized spores immersed or not immersed in liquid nitrogen, but when the spores were previously sterilized, a severe inhibition of germination was observed in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Liquid nitrogen; Rumohra; Storage. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042006000300016 |
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| Hiendlmeyer,Rosane; Randi,Aurea Maria. |
| Light is a limiting factor for fern stablishment because it controls germination of light sensitive spores. The aim of this work was to study the effect of light levels on spore germination in two ornamental ferns native to the Atlantic forest, under natural conditions. Cyathea delgadii is a tree fern and Blechnum brasiliense, a subarborescent fern. The effect of light levels was analyzed in April and July/2003, in Florianópolis, Santa Catarina state, Brazil. Sterilized spores were sown in Erlenmeyer flasks containing mineral culture medium with macronutrients, iron, and benomyl 0.01%. The Erlenmeyer flasks were kept in 50 cm³ boxes covered with black shade netting, which gave 5, 22, 42, and 62% natural light. Irradiance and temperature were scored daily... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ferns; Germination; Light levels; Photoinhibition; Spores. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062007000400015 |
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