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Economic feasibility of probiotic use in the diet of Nile tilapia, Oreochromis niloticus, during the reproductive period 2
Dias, Danielle de Carla; Furlaneto, Fernanda de Paiva Badiz; Sussel, Fábio Rosa; Tachibana, Leonardo; Gonçalves, Giovani Sampaio; Ishikawa, Carlos Massatoshi; Natori, Mariene Miyoko; Ranzani-Paiva, Maria José Tavares.
This work examines the economic advantages of probiotic use in the diet of Nile tilapia broodstock during the reproductive period. For this purpose, Bacillus subtilis was applied as a feed additive. The experimental design was completely randomized with three treatment groups: the T0 control (without probiotic), the T1 continuous probiotic intake, and the T2 alternate probiotic intake at a dose of 0.50 g kg-1 of feed (1010 CFU g-1) with four replicates. For the reproduction assay, 118 females and 48 males of Nile tilapia (proportion 4 males:9 females. hapa-1) (weight 527.65 g ± 185.98 g and length 30.16 cm ± 3.57 cm) were distributed into 12 hapas (3.5 × 2.0 × 1.5 m). Reproductive variables (spawning female percentage, egg production, and fry production)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus subtilis; Fish farming; Feed additive; Profitability; Spawning.
Ano: 2020 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/47960
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Isolation of Saprolegnia aenigmatica oomycetes and protocol for experimental infection of pacu (Piaractus mesopotamicus) 3
Carraschi, Silvia Patrícia; Garlich, Nathália; Souza-Pollo, Andressa; Pereira, Daniela Isabel Brayer; Cruz, Claudinei da; Ranzani-Paiva, Maria José Tavares.
 Successful disease treatment depends on molecular studies under indoor conditions with experimental infection protocols that facilitate understanding the disease and the drug`s efficacy. The internal transcribed spacer (ITS) region was sequenced from three isolates, which were identified as Saprolegnia aenigmatica. Subsequently, healthy fish were immunosuppressed with dexamethasone (1.2 mg kg-1) and descaled to the skin using a sharp scalpel. These individuals were isolated in individual aquariums maintained at 22°C. Individuals in one group were subcutaneously inoculated with 9,000 zoospores (DDZ treatment), a second group was exposed to oomycetes in water with three colonized baits (DDB), a third group was maintained in water without zoospores (DD), and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 5.06.03.00-0 oomycete; Zoosporangium; Oogonium; Saprolegniasis. Aquicultura.
Ano: 2018 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/38186
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