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| Celeghini,ECC; Arruda,RP; Albuquerque,R; Silva,FHA; Faria,DE; Andrade,AFC; Nascimento,J; Raphael,CF. |
| This experiment was designed with the objective of developing a simple, practical, and high repeatability technique for the simultaneous evaluation of the integrity of the plasmatic and acrosomal membranes, as well as funcional mitochondria of domestic fowl spermatozoa using an association of fluorescent probes. Four ejaculates (motility > 80% and abnormal morphology < 10%) from each of six Ross male broiler breeder (n=24) were diluted in TALP sperm medium (25x10(6) spermatozoa/mL) and split into two aliquots, and one of these aliquots was flash frozen in liquid nitrogen and thawed to damage all cellular membranes. Three treatments were prepared from these aliquots, with the following ratios of Fresh semen:Flash frozen semen: 100:0 (T100), 50:50... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fluorescent probes; Membranes; Rooster; Sperm. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2007000300001 |
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