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| Murtaza,B.N.; Bibi,A.; Rashid,M.U.; Khan,Y.I.; Chaudri,M.S.; Shakoori,A.R.. |
| The incidence of colorectal cancer (CRC) is increasing daily worldwide. Although different aspects of CRC have been studied in other parts of the world, relatively little or almost no information is available in Pakistan about different aspects of this disease at the molecular level. The present study was aimed at determining the frequency and prevalence of K ras gene mutations in Pakistani CRC patients. Tissue and blood samples of 150 CRC patients (64% male and 36% female) were used for PCR amplification of K ras and detection of mutations by denaturing gradient gel electrophoresis, restriction fragment length polymorphism analysis, and nucleotide sequencing. The K ras mutation frequency was found to be 13%, and the most prevalent mutations were found at... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Colorectal cancer; Kirsten rat sarcoma viral oncogene homologue; Mutation analysis; Risk factors for CRC; Cetuximab therapy. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000100035 |
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