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	Provedor de dados BABT (1) BJM (1) Autor Rasul,Samreen (2) Zia,Muhammad Anjum (2) Abbas,Rao Zahid (1) Riaz,Ayesha (1) Umbreen,Huma (1) Palavra-chave Antimicrobial (1) Aspergillus niger (1) Glucose aerodehydogenase (1) Glucose oxidase (1) Mutagenesis (1) Penicillium notatum (1) Production (1) Purification (1) Thermal stability (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2013 (2) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Evaluation of Antimicrobial Activity of Glucose Oxidase from Aspergillus niger EBL-A and Penicillium notatum BABT Zia,Muhammad Anjum; Riaz,Ayesha; Rasul,Samreen; Abbas,Rao Zahid. This work aimed to study the production and purification of glucose oxidase by Aspergillus niger and Penicillium notatum using corn steep liquor as the substrate and evaluate its antimicrobial activity for use in pharmaceutical and food industries. The enzyme was purified by ammonium sulfate precipitation (60-85%), DEAE-cellulose ion exchange and Sephadex G-200 size exclusion chromatography. The crude enzyme extracts of A. niger and P. notatum showed 2.32 and 5.53 U mg-1 specific activities, respectively, which after desalting was 15.52 and 12.05 U mg-1, and after ion exchange and gel filtration chromatography was 29.09 - 62 and 25.72 - 59.37 U mg-1 for A. niger and P. notatum, respectively. The antimicrobial activity was determined by disc diffusion... Tipo: Info:eu-repo/semantics/article Palavras-chave: Glucose oxidase; Antimicrobial; Aspergillus niger; Penicillium notatum. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000600010 Kinetic characterization of glucose aerodehydrogenase from Aspergillus niger EMS-150-F after optimizing the dose of mutagen for enhanced production of enzyme BJM Umbreen,Huma; Zia,Muhammad Anjum; Rasul,Samreen. In the present study enhanced production of glucose aerodehydrogenase from Aspergillus niger has been achieved after optimizing the dose of chemical mutagen ethyl methane sulfonate (EMS) that has not been reported earlier. Different doses of mutagen were applied and a strain was developed basing upon the best production. The selected strain Aspergillus niger EMS-150-F was optimized for nutrient requirements in order to produce enzyme through fermentation and the results showed the best yield at 2% corn steep liquor (CSL), 36 hours fermentation time, pH 5, 30°C temperature, 0.3% KH2PO4, 0.3% urea and 0.06% CaCO3. The enzyme was then purified and resulted in 57.88 fold purification with 52.12% recovery. On kinetic characterization, the enzyme showed optimum... Tipo: Info:eu-repo/semantics/article Palavras-chave: Glucose aerodehydogenase; Mutagenesis; Production; Purification; Thermal stability. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400012 Registros recuperados: 2 Primeira ... 1 ... Última

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