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| Sajidan,; Wulandari,Rita; Sari,Evy Novita; Ratriyanto,Adi; Weldekiros,Hailu; Greiner,Ralf. |
| ABSTRACTIn this study, 154 isolates capable of producing extracellular phytate-degrading activity were isolated from four soil samples from volcanic areas in Central Java, Indonesia. Six strains with high phytate-degrading activity were selected for strain identification and characterization of the corresponding phytate-degrading enzyme. Blast analysis of 16S rRNA gene sequences revealed high similarities for all the six isolates to reference sequences belonging to the genusBacillus. Isolates MS5, MC6, D10 and D16 showed 99% sequence identity toB. cereus, while isolate MC8 exhibited 99% sequence identity toB. aryabhatti and D6 99% sequence identity toB. psychrotolerans. The crude extracellular phytase preparations from the isolates showed following optimal... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: 16S rDNA; Bacillus sp. bacterial phytase phytate phytate-degrading enzyme. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000500711 |
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