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Induction of in vitro flowering in Capsicum frutescens under the influence of silver nitrate and cobalt chloride and pollen transformation 69
Sharma,Ashwani; Kumar,Vinod; Giridhar,Parvatam; Ravishankar,Gokare Aswathanarayana.
The influence of silver nitrate (AgNO3) and cobalt chloride (CoCl2) on shoot multiplication and in vitro flowering in Capsicum frutescens Mill. was investigated. Exogenous administration of AgNO3 and CoCl2 at a concentration of 30 µM resulted in the maximum tissue response in terms of shoot length and number of shoots after 45 days culturing on MS medium. Both silver nitrate (40 µM) and cobalt chloride (30 µM) influenced in vitro flowering after 25 and 45 days respectively. This is the first report on in vitro flowering in C. frutescens. The study also demonstrated successful transformation of pollen obtained from the in vitro flowers. Since capsicum is highly recalcitrant to in vitro plant regeneration, the results of the study may be highly useful in...
Tipo: Journal article Palavras-chave: Capsicum frutescens; Cobalt chloride; In vitro flowering; Pollen transformation; Regeneration; Silver nitrate.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000200011
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Isolation and characterization of hydrocarbon producing green alga Botryococcus braunii from Indian freshwater bodies 69
Dayananda,Chandrappa; Sarada,Ravi; Kumar,Vinod; Ravishankar,Gokare Aswathanarayana.
Samples of green colonial unicellular microalga Botryococcus braunii were collected from Bear Shola Falls at Kodaikanal (latitude 10.31 N and longitude 77.32 E), Tamil Nadu, India. Specimens were isolated, cultured and examined for its hydrocarbon content, morphological features and DNA structural resemblance with the known strain to confirm its identity. Inter simple sequence repeats (ISSR) finger printing revealed strong genetic similarity among the authentic strain (B. braunii N-836) and the Indian isolated strain (B. braunii CFTRI- Bb1) from Kodaikanal. The type of hydrocarbons produced by the Kodaikanal isolates were analyzed and identified as saturated hydrocarbons in the range of C21 to C33 by GCMS. Tetracosane and octacosane were found as the major...
Tipo: Journal article Palavras-chave: Biomass; Carbon dioxide; Carotenoids; Fatty acids; Inter simple sequence repeats; Saturated hydrocarbons.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000100008
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AgNO3: a potential regulator of ethylene activity and plant growth modulator 69
Kumar,Vinod; Parvatam,Giridhar; Ravishankar,Gokare Aswathanarayana.
The aim of this review is to critically analyze the role of silver nitrate (AgNO3) in modulating plant growth and development. In recent years, basic studies on ethylene regulation opened new vistas for applied research in the area of micro-propagation, somatic embryogenesis, in vitro flowering, growth promotion, fruit ripening, and sex expression. Silver nitrate has proved to be a very potent inhibitor of ethylene action and is widely used in plant tissue culture. Few properties of silver nitrate such as easy availability, solubility in water, specificity and stability make it very useful for various applications in exploiting plant growth regulation and morphogenesis in vivo and in vitro. Silver ion mediated responses seem to be involved in polyamines,...
Tipo: Journal article Palavras-chave: Calcium; Ethylene; Morphogenesis; Polyamines; Silver nitrate; Somatic embryogenesis.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000200008
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