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| Rayatpanah,Siavosh; Nanagulyan,Siranoush G; Alav,Seyed V; Razavi,Mohammad; Ghanbari-Malidarreh,Abbas. |
| Charcoal rot, caused by Macrophomina phaseolina (Tassi) Goid., is an economically important disease of oilseed plants in Northern Iran. Seventy isolates of M. phaseolina were obtained from different hosts, including soybean (Glycine max L.), and sunflower (Helianthus annuus L.) in the northern oilseed planting regions of Iran. RAPD-PCR amplification profiles, by using of six random OPA primers (kit A) showed polymorphisms among the isolates. The primer OPA-13 (5'_ CAGCACCCAC_3') amplified the genomic DNA of all isolates of M.phaseolina producing 4-12 bands of sizes between 0.25 and 2.3 kb. Unweighted pair-group method with arithmetic mean (UPGMA) analysis classified the isolates into the nine major groups with 64% similarity. Pathogenicity of isolates was... |
| Tipo: Journal article |
Palavras-chave: Charcoal rot; Oilseed plants; Disease index. |
| Ano: 2012 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392012000100007 |
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