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Communities of arbuscular mycorrhizal fungi in arable soils are not necessarily low in diversity Organic Eprints
Hijri, Isabelle; Sýkorová, Zuzana; Oehl, Fritz; Ineichen, Kurt; Mäder, Paul; Wiemken, Andres; Redecker, Dirk.
Communities of arbuscular mycorrhizal fungi (AMF) in five agricultural field sites of different management intensities were studied. Variable regions of the ribosomal RNA genes were used to detect and identify AMF directly within colonized roots. Roots from a continuous maize monoculture showed low AMF diversity, in agreement with previous reports on molecular diversity of AMF in agricultural soils. In contrast, a substantially higher diversity of AMF was found throughout the long term `DOK' field experiment, where organic and conventional agricultural practices have been compared side by side since 1978. In this experiment, a 7-year crop rotation is performed under lower levels of inorganic fertilizer input and chemical pest control. These results are in...
Tipo: Journal paper Palavras-chave: Soil biology; Soil quality.
Ano: 2007 URL: http://orgprints.org/13462/1/hijri%2Detal%2D2006%2Dmolecular_ecology%2Dvol15%2D2277%2D89.pdf
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Diversity of mitochondrial large subunit rDNA haplotypes of Glomus intraradices in two agricultural field experiments and two semi-natural grasslands Organic Eprints
Börstler , Boris; Thiéry, Odile; Sýkorová, Zuzana; Berner, Alfred; Redecker, Dirk.
Glomus intraradices, an arbuscular mycorrhizal fungus (AMF), is frequently found in a surprisingly wide range of ecosystems all over the world. It is used as model organism for AMF and its genome is being sequenced. Despite the ecological importance of AMF, little has been known about their population structure, because no adequate molecular markers have been available. In the present study we analyse for the first time the intraspecific genetic structure of an AMF directly from colonized roots in the field. A recently developed PCR-RFLP approach for the mitochondrial rRNA large subunit gene (mtLSU) of these obligate symbionts was used and complemented by sequencing and primers specific for a particularly frequent mtLSU haplotype. We analysed root samples...
Tipo: Journal paper Palavras-chave: Breeding; Genetics and propagation.
Ano: 2010 URL: http://orgprints.org/17898/1/B%C3%B6rstler-etal-2010-molecular-ecology.pdf
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