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Production of hydrolytic enzymes by the plant pathogenic fungus Myrothecium verrucaria in submerged cultures BJM
Moreira,Fabiana Guillen; Reis,Simone dos; Costa,Maria Aparecida Ferreira; Souza,Cristina Giatti Marques de; Peralta,Rosane Marina.
The capability of the plant pathogenic fungus Myrothecium verrucaria to produce extracellular hydrolytic enzymes in submerged cultures was studied using several substrates. The fungus was able to produce different depolymerases and glycosidases, being xylanase, pectinase and protease the most important. Lipase was found in cultures developed in the presence of olive oil, while protease activity was detected in all cultures. Xylanase and pectinase were optimally active at pH 4.5-5.5, while protease was active in a large range of pH 3.5 to 11.0. All three enzymes were maximally active at 40ºC and they were stable for several hours at temperature up to 50ºC.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Carbohydrases; Hydrolytic enzymes; Myrothecium verrucaria; Protease.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000100002
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