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Identification of yeasts Isolated from processed and frozen cocoa (Theobroma cacao) pulp for wine production BABT
Trindade,Rita de Cássia; Resende,Maria Aparecida de; Barreto,Eriana Gomes Serpa; Mendes,Taniella de Carvalho; Rosa,Carlos Augusto.
The alternative use of cocoa (Theobroma cacao) for wine production was tested. The pulp samples, obtained from Formosa farm, Itacaré, Brazil, were diluted, homogenized and inoculated on Sabouraud dextrose agar medium (SDA) and incubated at 28º C for 5-8 days. Selected colonies were tested for the ability to ferment cocoa pulp and divided into fermentative, non-fermentative and weak/late fermentative species. Isolates characterized as fermentative were further tested in a small-scale wine production plant and identified. Species from the genus Brettanomyces constituted the main fermentative yeasts, with the exception of two Kloeckera apis samples. The final wine product was normally pale or clear, making clarification unnecessary, and with a sweet or dry...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Yeast; Cocoa pulp; Fermentation; Wine production; Brettanomyces sp.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000300013
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Antifungal properties of plants used in Brazilian traditional medicine against clinically relevant fungal pathogens BJM
Johann,Susana; Pizzolatti,Moacir G.; Donnici,Cláudio L.; Resende,Maria Aparecida de.
Antifungal properties of extracts from eight Brazilian plants traditionally used in popular Brazilian medicine were tested against five clinically relevant Candida species, Cryptococcus neoformans, and Sporothrix schenckii. Results demonstrate that almost all extracts exhibited antifungal activity, at least against one of the microorganisms tested. The ethanolic extract from the leaves of Schinus terebinthifolius exhibited potential antifungal activity against C. glabrata and S. schenckii. Preliminary phytochemical analysis of extract from S. terebinthifolius showed the presence of biologically active compounds, namely saponins, flavonoids, triterpenes, steroids and tannins.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antifungal properties; Medicinal plants; Susceptibility test.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400010
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Antifungal susceptibility profile of Trichosporon isolates: correlation between CLSI and etest methodologies BJM
Lemes,Raquel M.L.; Lyon,Juliana P.; Moreira,Leonardo M.; Resende,Maria Aparecida de.
The aim of the present study was to evaluate the antifungal susceptibility profile of Trichosporon species isolated from different sources employing the Clinical and Laboratory Standards Institute (CLSI) method and E-test method. Thirty-four isolates of Trichosporon spp. and six CBS reference samples were tested for their susceptibility to Amphotericin B, 5-flucytosine, Fluconazole, Itraconazole, Voriconazole and Terbinafine. All species showed high Minimun Inhibitory Concentrations (MIC) for Itraconazole and susceptibility to Fluconazole, The comparison among the results obtained by the CLSI method and E-test revealed larger discrepancies among 5-flucytosine and Itraconazole. The present work provides epidemiological data that could influence therapeutic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Trichosporon spp; Antifungal drugs; CLSI; E-test.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200008
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Candida albicans: genotyping methods and clade related phenotypic characteristics BJM
Lyon,Juliana P.; Moraes,Karen C.M.; Moreira,Leonardo M.; Aimbire,Flávio; Resende,Maria Aparecida de.
Several molecular methods, such as Southern blotting hybridization, Multilocus Sequence Typing, and DNA microsatellite analysis, have been employed to genotype Candida albicans. The genotype analysis allows to group strains in clades, that is, a group composed of one ancestor and its descendants. These genotype studies demonstrate that clades distribution is influenced by geographic area as well as that antifungal resistance is associated with particular clades. These findings suggested that C. albicans reproduces mainly in a clonal manner, with certain degree of DNA microevolution. Additionally, virulence factors and site of isolation have also been associated with clade specificity. The present article is a brief review about the methods used for Candida...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Candida albicans; Clonal reproduction; Microevolution; Genotyping systems; Phenotypic characteristics.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400003
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