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| Rezende,A.C.S.; Vieira,A.S.; Rogério,F.; Rezende,L.F.; Boschero,A.C.; Negro,A.; Langone,F.. |
| Ciliary neurotrophic factor (CNTF) is a cytokine that plays a neuroprotective role in relation to axotomized motoneurons. We determined the effect of daily subcutaneous doses of CNTF (1.2 µg/g for 5 days; N = 13) or PBS (N = 13) on the levels of mRNA for Bcl-2 and Bax, as well as the expression and inter-association of Bcl-2 and Bax proteins, and the survival of motoneurons in the spinal cord lumbar enlargement of 2-day-old Wistar rats after sciatic nerve transection. Five days after transection, the effects were evaluated on histological and molecular levels using Nissl staining, immunoprecipitation, Western blot analysis, and reverse transcriptase-polymerase chain reaction. The motoneuron survival ratio, defined as the ratio between the number of... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Ciliary neurotrophic factor; Bax; Neonatal rats; Axotomy; Motoneuron; Immunoprecipitation. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001100013 |
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