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Endo-β-mannanase enzyme activity in the structures of Coffea arabica L. seeds under different types of processing and drying Ciência Rural
Ferreira,Valquíria Fátima; Ricaldoni,Marcela Andreotti; Rosa,Sttela Dellyzete Veiga Franco da; Figueiredo,Madeleine Alves de; Coelho,Stefânia Vilas Boas; Fantazzini,Tatiana Botelho.
ABSTRACT: Enzymes play a fundamental role in degradation of molecules during seed germination, development, and deterioration. Endo-β-mannanase is one of the main enzymes responsible for hydrolysis of mannans in the endosperm during germination of coffee seeds through its action in hydrolytic degradation of cell walls and in weakening the structures of the endosperm that surround the embryo, allowing radicle emergence. The aim of this study was to determine the activity of the endo-β-mannanase enzyme in the structures of coffee seeds for the purpose of assessing the relationship between this activity and the physiological quality of the seeds under different processing and drying methods. Coffea arabica L. fruit in the cherry maturity stage was subjected...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Metabolic reactions; Hydrolysis of mannans; Coffea arabica L..
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018001200200
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Physiological, biochemical, and ultrastructural aspects of Coffea arabica L. seeds under different cryopreservation protocols Ciência e Agrotecnologia
Figueiredo,Madeleine Alves de; Rosa,Sttela Dellyzete Veiga Franco da; Ricaldoni,Marcela Andreotti; Pereira,Cristiane Carvalho; Coelho,Stefânia Vilas Boas; Silva,Luciano Coutinho.
ABSTRACT Cryopreservation is a technique that may potentially conserve the germplasm of species of the Coffea genus for an indeterminate time. The aim of this study was to evaluate the physiological, biochemical and ultrastructural characteristics of cryopreserved seeds of Coffea arabica L., cultivar Catucaí amarelo IAC 62, which was subjected to different protocols regarding dehydration, precooling, cooling, rewarming and cathode water use. According to each protocol, the seeds were subjected to fast or slow drying to moisture contents of 17 or 20% (wet basis), cooled in different ways, and then immersed in liquid nitrogen for 24 hours. Different rewarming times in a water bath were also used. Physiological, biochemical and ultrastructural analyses were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cathodic water; Dehydration; Long-term conservation; Ultrafast cooling..
Ano: 2021 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542021000100203
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