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| Mori,Enio; Lara,Maria do Carmo C.S.H.; Cunha,Elenice M.S.; Villalobos,Eliana M.C.; Mori,Claudia M.C.; Soares,Rodrigo M.; Brandão,Paulo E.; Fernandes,Wilson R.; Richtzenhain,Leonardo J.. |
| Partial nucleotide sequences of ORF72 (glycoprotein D, gD), ORF64 (infected cell protein 4, ICP4) and ORF30 (DNA polymerase) genes were compared with corresponding sequences of EHV-1 reference strains to characterize the molecular variability of Brazilian strains. Virus isolation assays were applied to 74 samples including visceral tissue, total blood, cerebrospinal fluid (CSF) and nasal swabs of specimens from a total of 64 animals. Only one CSF sample (Iso07/05 strain) was positive by virus isolation in cell culture. EHV-1 Iso07/05 neurologic strain and two abortion visceral tissues samples (Iso11/06 and Iso33/06) were PCR-positive for ORF33 (glycoprotein B, gB) gene of EHV-1. A sequence analysis of the ORF72, ORF64 and ORF30 genes from three EHV-1... |
| Tipo: Info:eu-repo/semantics/report |
Palavras-chave: Equid herpesvirus type 1; Equid Brazilian herpesvirus; ICP4 gene (ORF64); Glycoprotein D gene (ORF72); DNA polymerase gene (ORF30). |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200565 |
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| Horta,Mauricio C.; Scott,Fabio B.; Correia,Thaís R.; Fernandes,Julio I.; Richtzenhain,Leonardo J.; Labruna,Marcelo B.. |
| The present study evaluated the rickettsial infection in a laboratory colony of cat fleas, Ctenocephalides felis felis (Bouche) in Brazil. All flea samples (30 eggs, 30 larvae, 30 cocoons, 30 males, and 30 females) tested by polymerase chain reaction (PCR) were shown to contain rickettsial DNA. PCR products, corresponding to the rickettsial gltA, htrA, ompA and ompB gene partial sequences were sequenced and showed to correspond to Rickettsia felis, indicating that the flea colony was 100% infected by R. felis. The immunofluorescence assay (IFA) showed the presence of R. felis-reactive antibodies in blood sera of 7 (87.5%) out of 8 cats that were regularly used to feed the flea colony. From 15 humans that used to work with the flea colony in the laboratory,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rickettsia felis; Flea; Ctenocephalides felis felis; Cats; Humans. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300035 |
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