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	Provedor de dados Rev. Bras. Ciênc. Avic. (1) Autor Meireles,MV (1) Riera,RF (1) Roberto,LO (1) Palavra-chave Diagnosis (1) Eimeria mitis (1) Eimeria praecox (1) Epidemiology (1) Polymerase chain reaction (1) Tipo do documento Info:eu-repo/semantics/article (1) Ano 2004 (1) País Brazil (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Identification of Eimeria mitis and Eimeria praecox in broiler feces using polymerase chain reaction Rev. Bras. Ciênc. Avic. Meireles,MV; Roberto,LO; Riera,RF. There are few reports concerning the epidemiology of Eimeria praecox and Eimeria mitis in Brazil. In the present experiment, the polymerase chain reaction (PCR) was used to identify these species in 156 samples of broiler chicken feces from several Brazilian states and the Federal District. Oocysts present in feces samples were purified by sodium chloride flotation followed by addition of DNAzol reagent (Invitrogen®) for extraction of genomic DNA. DNA was precipitated and stored following DNAzol reagent manufacture's instructions. The primers and PCR conditions were as described by Schnitzler et al. (1999). In the 156 field samples analyzed by PCR, 70 and 45 were positive for E. praecox and E. mitis, respectively. In this study we have shown that DNA... Tipo: Info:eu-repo/semantics/article Palavras-chave: Diagnosis; Eimeria mitis; Eimeria praecox; Epidemiology; Polymerase chain reaction. Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2004000400010 Registros recuperados: 1 Primeira ... 1 ... Última

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