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Detailed investigation of hydrothermal site Rainbow, Mid-Atlantic Ridge, 36°13’N: Cruise MoMARDream ArchiMer
Dyment, J.; Bissessur, D.; Bucas, Karenn; Cueff-gauchard, Valerie; Durand, Lucile; Fouquet, Yves; Gaill, F.; Gente, P.; Hoise, E.; Ildefonse, B.; Konn, Cecile; Lartaud, F.; Le Bris, Nadine; Musset, G.; Nunes, A.; Renard, J.; Riou, V.; Tasiemski, A.; Thibaud, R.; Torres, P.; Yatheesh, V.; Vodjdani, I.; Zbinden, M..
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Ano: 2009 URL: http://archimer.ifremer.fr/doc/00385/49651/50164.pdf
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Influence of CH4 and H2S availability on symbiont distribution, carbon assimilation and transfer in the dual symbiotic vent mussel Bathymodiolus azoricus ArchiMer
Riou, V.; Halary, S.; Duperron, S.; Bouillon, S.; Elskens, M.; Bettencourt, R.; Santos, R. S.; Dehairs, F.; Colaco, A..
High densities of mussels of the genus Bathymodiolus are present at hydrothermal vents of the Mid-Atlantic Ridge. It was previously proposed that the chemistry at vent sites would affect their sulphide- and methane-oxidizing endosymbionts' abundance. In this study, we confirmed the latter assumption using fluorescence in situ hybridization on Bathymodiolus azoricus specimens maintained in a controlled laboratory environment at atmospheric pressure with one, both or none of the chemical substrates. A high level of symbiosis plasticity was observed, methane-oxidizers occupying between 4 and 39% of total bacterial area and both symbionts developing according to the presence or absence of their substrates. Using (HCO3-)-C-13 in the presence of sulphide, or...
Tipo: Text Palavras-chave: Mid-atlantic ridge; Hydrocarbon-seep mussel; Deep-sea mussels; Hydrothermal-vent; Puteoserpentis bivalvia; Mytilidae; Methane; Endosymbionts; Bacteria; Metabolism.
Ano: 2008 URL: https://archimer.ifremer.fr/doc/00207/31865/30271.pdf
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Tracing carbon assimilation in endosymbiotic deep-sea hydrothermal vent Mytilid fatty acids by C-13-fingerprinting ArchiMer
Riou, V.; Bouillon, S.; Serrao Santos, R.; Dehairs, F.; Colaco, A..
Bathymodiolus azoricus mussels thrive at Mid-Atlantic Ridge hydrothermal vents, where part of their energy requirements are met via an endosymbiotic association with chemolithotrophic and methanotrophic bacteria. In an effort to describe phenotypic characteristics of the two bacterial endosymbionts and to assess their ability to assimilate CO2, CH4 and multi-carbon compounds, we performed experiments in aquaria using C-13-labeled NaHCO3 (in the presence of H2S), CH4 or amino-acids and traced the incorporation of C-13 into total and phospholipid fatty acids (tFA and PLFA, respectively). 14:0; 15:0; 16:0; 16:1(n - 7) c+t; 18:1(n - 13) c+t and (n - 7) c+t; 20:1(n - 7); 20:2(n - 9,15); 18:3(n - 7) and (n - 5,10,13) PLFA were labeled in the presence of...
Tipo: Text Palavras-chave: Mid atlantic ridge; Mussel bathymodiolus azoricus; Methanotrophic bacteria; Marine invertebrates; Diversity; Organic compounds; Lipid composition; Dual symbiosis; Methane; Biomarkers.
Ano: 2010 URL: https://archimer.ifremer.fr/doc/00207/31868/30275.pdf
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