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	Provedor de dados Electron. J. Biotechnol. (2) Autor Rivera-Domínguez,Marisela (2) Aispuro-Hernández,Emmanuel (1) Astorga-Cienfuegos,Karen Rosalinda (1) García-Baldenegro,Claudia Vanessa (1) González-Aguilar,Gustavo Adolfo (1) Islas-Osuna,María (1) Martínez-Téllez,Miguel Ángel (1) Tiznado-Hernández,Martin Ernesto (1) Vargas-Arispuro,Irasema (1) Mais... Palavra-chave Cold treatment (1) Defence proteins (1) Freeze-dried (1) Genetic expression (1) Jasmonic acid methyl ester (1) Papaya fruit (1) RNA integrity number (1) RT-qPCR (1) Vitis vinifera L (1) Woody tissue (1) Mais... Tipo do documento Journal article (2) Ano 2015 (1) 2012 (1) País Chile (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Total RNA quality of lyophilized and cryopreserved dormant grapevine buds Electron. J. Biotechnol. García-Baldenegro,Claudia Vanessa; Vargas-Arispuro,Irasema; Islas-Osuna,María; Rivera-Domínguez,Marisela; Aispuro-Hernández,Emmanuel; Martínez-Téllez,Miguel Ángel. Background Plant tissues must be preserved in their collection state, especially for genome-wide expression profile studies. Lyophilization is a feasible, affordable tool when fresh tissues cannot be shipped at ultralow temperatures from their origin to the place of analysis. In this study, the total RNA quality of dormant grapevine buds (Vitis vinifera L. cv. ‘Flame Seedless’) of freeze-dried samples stored at room temperature conditions was evaluated and compared to that of cryopreserved (-80°C) grapevine buds. Results Good yield and quality of RNA were obtained from freeze-dried dormant buds stored at room temperature for 0, 3 and 6 weeks after they were lyophilized. Further experiments confirmed that the extracted total RNA could be used for actin and... Tipo: Journal article Palavras-chave: Freeze-dried; RNA integrity number; RT-qPCR; Vitis vinifera L; Woody tissue. Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000200012 Induction of the expression of defence genes in Carica papaya fruit by methyl jasmonate and low temperature treatments Electron. J. Biotechnol. Rivera-Domínguez,Marisela; Astorga-Cienfuegos,Karen Rosalinda; Tiznado-Hernández,Martin Ernesto; González-Aguilar,Gustavo Adolfo. The defence mechanisms that are activated by methyl jasmonate (MJ) in fruits are not well understood. In this work, we studied the expression of defence genes in papaya fruit that are induced by the exposure to MJ and/or low temperatures. The papaya fruits ‘Maradol’ were randomly divided into two groups: one group was the untreated control and the other was treated with 10-4 M of MJ. Half of the fruits from each of the two groups were stored after treatment for 5 days at 5ºC and 2 days at 20ºC. We studied the expression levels of the pdf1.1 and pdf1.2 genes by amplification from expression libraries created from the pulp and skin tissues of the papaya fruit. As a reference, the mRNA level of the 18s ribosomal gene was used. In the skin... Tipo: Journal article Palavras-chave: Cold treatment; Defence proteins; Genetic expression; Jasmonic acid methyl ester; Papaya fruit. Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000500006 Registros recuperados: 2 Primeira ... 1 ... Última

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