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Hank’s balanced salt solution: an alternative resuspension medium to label autologous leukocytes. Experience in inflammatory bowel disease BABT
Martin-Comin,Joseph; Cardoso,Valbert Nascimento; Plaza,Pedro; Roca,Manoel.
In this work Hank's balanced salt solution (HBSS) has been used, as resuspension medium, instead of leukocyte poor plasma (LPP) to label autologous white blood cells in 28 patients with suspicion af active inflammatory bowel disease.Labelled cells were reinjected and anterior and caudo-craneal views were obtained at 30 min, 2 h and 6 h p.i. Regions of interest were outlined on liver, spleen, lung, bone marrow (spine), background and lesions and the organ/background activity ratios were calculated in all scans. Patients were classified into 2 groups: Group 1: LPP, 30 patients and Groups 2: HBSS, 28 patients. Labelling efficiency was higher in HBBS group (89.0 ± 3.2 %) than in the LPP group (6.5 ± 6.3%). Organ/background activity ratios were similar in both...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 99mTechnetium; Leukocytes; Hank' s solution; Inflammatory.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000500006
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