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Metabolomic Profiles of Dinophysis acuminata and Dinophysis acuta Using Non-Targeted High-Resolution Mass Spectrometry: Effect of Nutritional Status and Prey ArchiMer
Garcia-portela, Maria; Reguera, Beatriz; Sibat, Manoella; Altenburger, Andreas; Rodriguez, Francisco; Hess, Philipp.
Photosynthetic species of the genus Dinophysis are obligate mixotrophs with temporary plastids (kleptoplastids) that are acquired from the ciliate Mesodinium rubrum, which feeds on cryptophytes of the Teleaulax-Plagioselmis-Geminigera clade. A metabolomic study of the three-species food chain Dinophysis-Mesodinium-Teleaulax was carried out using mass spectrometric analysis of extracts of batch-cultured cells of each level of that food chain. The main goal was to compare the metabolomic expression of Galician strains of Dinophysis acuminata and D. acuta that were subjected to different feeding regimes (well-fed and prey-limited) and feeding on two Mesodinium (Spanish and Danish) strains. Both Dinophysis species were able to grow while feeding on both...
Tipo: Text Palavras-chave: Dinophysis physiology; Lipophilic toxins; Non-targeted analysis; Metabolomics.
Ano: 2018 URL: http://archimer.ifremer.fr/doc/00439/55077/56508.pdf
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Phylogenetic and morphological characterisation of the green algae infecting blue mussel Mytilus edulis in the North and South Atlantic oceans ArchiMer
Rodriguez, Francisco; Feist, Stephen; Guillou, Laure; Harkestad, Lisbeth; Bateman, Kelly; Renault, Tristan; Mortensen, Stein.
Blue mussels Mytilus edulis with shell deformations and green pustules containing parasitic algae were collected at 3 coastal sites (Burøy, Norway; Bockholm, Denmark; Goose Green, Falkland Islands). A comparative study, including mussel histopathology, algal morphology, ultrastructure and phylogenetic position was performed. Green pustules were mainly located in the posterior portion of the mantle and gonad tissues and the posterior adductor muscle. Electron microscopy confirmed the presence of algal cells with similar morphology to Coccomyxa parasitica. Algae were oval shaped with a single nucleus and chloroplast, 1 or 2 mitochondria and a dense granular cytoplasm with a lipid inclusion body, Golgi apparatus and small vesicles. Partial small subunit (SSU)...
Tipo: Text Palavras-chave: Phylogeny; Parasitic alga; Mytilus edulis chilensis; Mytilus edulis; Coccomyxa parasitica; Blue mussels.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4581.pdf
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