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PCR-mediated recombination in development of microsatellite markers: mechanism and implications Genet. Mol. Biol.
Roratto,Paula A.; Buchmann,Darine; Santos,Sandro; Bartholomei-Santos,Marlise L..
Protocols for microsatellite-enrichment libraries have been widely applied to several species in order to supply the most informative molecular markers for population and inbreeding studies. One drawback of these protocols is the ratio of designed primer pairs that fail to amplify the expected fragment, even after exhaustive optimization attempts. A possible cause of unsuccessful microsatellite primers may be that such loci are artifacts resulting from chimeric PCR products, instead of real genomic sequences. The microsatellite-enriched library constructed for Aegla longirostri (Crustacea, Decapoda, Anomura) showed that 29% of sequenced clones were chimeric products because these sequences shared one of the flanking regions around the same repeat motif but...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Chimeric PCR product; Microsatellite isolation; Recombination; Simple sequence repeats (SSR).
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000100012
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